Curated Information
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Curated Information Page
PubMed Id: 21151116 
Estève PO, et al. (2011) A methylation and phosphorylation switch between an adjacent lysine and serine determines human DNMT1 stability. Nat Struct Mol Biol 18, 42-8 21151116
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
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K142-m1 - DNMT1 (human)
Orthologous residues
DNMT1 (human): K142‑m1, DNMT1 iso2 (human): K142‑m1, DNMT1 (mouse): K139‑m1, DNMT1 (rat): K139‑m1
 Methods used to characterize site in vivo modification-specific antibody, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
 Enzymes shown to modify site in vitro
Type Enzyme
Downstream Regulation
 Effect of modification (function):  phosphorylation, protein degradation, ubiquitination
 Comments:  inhibits S143 phosphorylation

S143-p - DNMT1 (human)
Orthologous residues
DNMT1 (human): S143‑p, DNMT1 iso2 (human): S143‑p, DNMT1 (mouse): S140‑p, DNMT1 (rat): S140‑p
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human, monkey
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (human) inhibition of upstream enzyme, transfection of dominant-negative enzyme, siRNA inhibition of enzyme, transfection of constitutively active enzyme
KINASE Akt1 (human) co-immunoprecipitation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
peptide agonist increase
siRNA decrease
LY294002 decrease
calyculin A increase
insulin increase
Downstream Regulation
 Effect of modification (function):  methylation, protein stabilization
 Comments:  inhibits K142 methylation

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