Curated Information
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Curated Information Page
PubMed Id: 21123648 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Kaneko S, et al. (2010) Phosphorylation of the PRC2 component Ezh2 is cell cycle-regulated and up-regulates its binding to ncRNA. Genes Dev 24, 2615-20 21123648
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T339-p - EZH2 (mouse)
Orthologous residues
EZH2 (human): T339‑p, EZH2 iso2 (human): T344‑p, EZH2 (mouse): T339‑p, EZH2 (rat): T339‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293F (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

T345-p - EZH2 (mouse)
Orthologous residues
EZH2 (human): T345‑p, EZH2 iso2 (human): T350‑p, EZH2 (mouse): T345‑p, EZH2 (rat): T345‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293F (epithelial), ES (stem), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
olomoucine decrease
roscovitine decrease
etoposide no change compared to control
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
RNA Induces
 Comments:  enhances interaction with HOTAIR noncoding RNA (ncRNA0

T487-p - EZH2 (mouse)
Orthologous residues
EZH2 (human): T487‑p, EZH2 iso2 (human): T492‑p, EZH2 (mouse): T487‑p, EZH2 (rat): T487‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293F (epithelial), ES (stem), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)


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