Curated Information
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Curated Information Page
PubMed Id: 20861461 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Chessa TA, et al. (2010) Phosphorylation of threonine 154 in p40phox is an important physiological signal for activation of the neutrophil NADPH oxidase. Blood 116, 6027-36 20861461
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T154-p - p40phox (mouse)
Orthologous residues
p40phox (human): T154‑p, p40phox iso2 (human): T154‑p, p40phox (mouse): T154‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  neutrophil, neutrophil [PKCD (mouse), homozygous knockout]
 Cellular systems studied:  primary cells
 Species studied:  mouse
 Comments:  bone marrow derived neutrophils
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKCD (mouse) activation of upstream enzyme, pharmacological activator of upstream enzyme, genetic knockout/knockin of upstream enzyme, pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol ester increase
bisindolylmaleimide phorbol ester inhibit treatment-induced increase
Go 6976 phorbol ester no effect upon treatment-induced increase
Go 6983 phorbol ester inhibit treatment-induced increase
wortmannin phorbol ester no effect upon treatment-induced increase
S. aureus increase opsonized S aureus
Go 6976 S. aureus no effect upon treatment-induced increase
bisindolylmaleimide S. aureus inhibit treatment-induced increase
Go 6983 S. aureus inhibit treatment-induced increase
wortmannin S. aureus inhibit treatment-induced increase
RBCs increase sheep RBCs
bisindolylmaleimide RBCs inhibit treatment-induced increase
Go 6976 RBCs inhibit treatment-induced increase
Go 6983 RBCs inhibit treatment-induced increase
wortmannin RBCs no effect upon treatment-induced increase
fMLP increase
bisindolylmaleimide fMLP inhibit treatment-induced increase
Go 6976 fMLP inhibit treatment-induced increase
wortmannin fMLP inhibit treatment-induced increase mild inhibition
Downstream Regulation
 Effect of modification (function):  intracellular localization
 Comments:  essential for NADPH oxidase activity; translocation to S aureus phagosomes

S315-p - p40phox (mouse)
Orthologous residues
p40phox (human): S315‑p, p40phox iso2 (human): , p40phox (mouse): S315‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  neutrophil
 Cellular systems studied:  primary cells
 Species studied:  mouse
 Comments:  bone marrow derived neutrophils


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