Britton DJ, et al. (2008) A novel serine phosphorylation site detected in the N-terminal domain of estrogen receptor isolated from human breast cancer cells. J Am Soc Mass Spectrom 19, 729-40 18367407

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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S104-p - ER-alpha (human) ▲

Modsite: FPPLNsVsPsPLMLL SwissProt Entrez-Gene Orthologous residues ER‑alpha (human): S104‑p, ER‑alpha (mouse): S108‑p, ER‑alpha (rat): S109‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  MCF-7 (breast cell) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes estradiol increase EGF no change compared to control slight increase

S106-p - ER-alpha (human) ▲

Modsite: PLNsVsPsPLMLLHP SwissProt Entrez-Gene Orthologous residues ER‑alpha (human): S106‑p, ER‑alpha (mouse): S110‑p, ER‑alpha (rat): S111‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  MCF-7 (breast cell) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes estradiol increase EGF no change compared to control slight increase

S118-p - ER-alpha (human) ▲

Modsite: LHPPPQLsPFLQPHG SwissProt Entrez-Gene Orthologous residues ER‑alpha (human): S118‑p, ER‑alpha (mouse): S122‑p, ER‑alpha (rat): S123‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, phospho-antibody, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  MCF-7 (breast cell) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes estradiol increase EGF increase

S154-p - ER-alpha (human) ▲

Modsite: PAFYRPNsDNRRQGG SwissProt Entrez-Gene Orthologous residues ER‑alpha (human): S154‑p, ER‑alpha (mouse): S158‑p, ER‑alpha (rat): S159‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, phospho-antibody, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  MCF-7 (breast cell) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes EGF increase estradiol increase

S167-p - ER-alpha (human) ▲

Modsite: GGRERLAsTNDkGSM SwissProt Entrez-Gene Orthologous residues ER‑alpha (human): S167‑p, ER‑alpha (mouse): S171‑p, ER‑alpha (rat): S172‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, phospho-antibody, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  MCF-7 (breast cell) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes estradiol increase EGF increase