Curated Information
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Curated Information Page
PubMed Id: 10205168 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Mason CS, et al. (1999) Serine and tyrosine phosphorylations cooperate in Raf-1, but not B-Raf activation. EMBO J 18, 2137-48 10205168
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S483-p - B-Raf (mouse) ▲
Orthologous residues
B‑Raf (human): S446‑p, B‑Raf (mouse): S483‑p, B‑Raf iso3 (mouse): ‑, B‑Raf (rat): S483‑p, B‑Raf (chicken): S486‑p, B‑Raf (quail): S486‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast), PC-12 (chromaffin)
 Cellular systems studied:  cell lines
 Species studied:  monkey, rat

S338-p - c-Raf (mouse) ▲
Orthologous residues
c‑Raf (human): S338‑p, c‑Raf (mouse): S338‑p, c‑Raf (rat): S338‑p, c‑Raf (chicken): S338‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase
PDGF increase
phorbol ester increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced

Y341-p - c-Raf (mouse) ▲
Orthologous residues
c‑Raf (human): Y341‑p, c‑Raf (mouse): Y341‑p, c‑Raf (rat): Y341‑p, c‑Raf (chicken): Y341‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey, mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Src (mouse) transfection of constitutively active enzyme, phospho-antibody
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase
PDGF increase
phorbol ester increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced


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