Curated Information
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Curated Information Page
PubMed Id: 10550055 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Cortez D, Wang Y, Qin J, Elledge SJ (1999) Requirement of ATM-dependent phosphorylation of brca1 in the DNA damage response to double-strand breaks. Science 286, 1162-6 10550055
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S1189-p - BRCA1 (human) ▲
Orthologous residues
BRCA1 (human): S1189‑p, BRCA1 iso6 (human): ‑, BRCA1 (mouse): S1152‑p, BRCA1 (rat): S1153‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, electrophoretic mobility shift, mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ATM (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
ATM (human) Induces co-immunoprecipitation

S1423-p - BRCA1 (human) ▲
Orthologous residues
BRCA1 (human): S1423‑p, BRCA1 iso6 (human): S320‑p, BRCA1 (mouse): N1379‑p, BRCA1 (rat): S1380‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, electrophoretic mobility shift, mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ATM (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
ATM (human) Induces co-immunoprecipitation

S1457-p - BRCA1 (human) ▲
Orthologous residues
BRCA1 (human): S1457‑p, BRCA1 iso6 (human): S353‑p, BRCA1 (mouse): S1413‑p, BRCA1 (rat): S1414‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, electrophoretic mobility shift, mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ATM (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
ATM (human) Induces co-immunoprecipitation

S1497-p - BRCA1 (human) ▲
Orthologous residues
BRCA1 (human): S1497‑p, BRCA1 iso6 (human): S393‑p, BRCA1 (mouse): S1454‑p, BRCA1 (rat): S1455‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, electrophoretic mobility shift, mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ATM (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
ATM (human) Induces co-immunoprecipitation

S1524-p - BRCA1 (human) ▲
Orthologous residues
BRCA1 (human): S1524‑p, BRCA1 iso6 (human): S420‑p, BRCA1 (mouse): S1481‑p, BRCA1 (rat): S1482‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, electrophoretic mobility shift, mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ATM (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
ATM (human) Induces co-immunoprecipitation

S1542-p - BRCA1 (human) ▲
Orthologous residues
BRCA1 (human): S1542‑p, BRCA1 iso6 (human): S438‑p, BRCA1 (mouse): S1495‑p, BRCA1 (rat): C1496‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, electrophoretic mobility shift, mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ATM (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
ATM (human) Induces co-immunoprecipitation


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