Curated Information
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PubMed Id: 21042876 
Soliman GA, Acosta-Jaquez HA, Fingar DC (2010) mTORC1 inhibition via rapamycin promotes triacylglycerol lipolysis and release of free fatty acids in 3T3-L1 adipocytes. Lipids 45, 1089-100 21042876
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S557-p - HSL (mouse)
Orthologous residues
HSL (human): S853‑p, HSL iso2 (human): S552‑p, HSL (mouse): S557‑p, HSL iso3 (mouse): S870‑p, HSL (rat): S863‑p, HSL iso2 (rat): S563‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
isoproterenol increase
rapamycin isoproterenol augment treatment-induced increase

S559-p - HSL (mouse)
Orthologous residues
HSL (human): S855‑p, HSL iso2 (human): S554‑p, HSL (mouse): S559‑p, HSL iso3 (mouse): S872‑p, HSL (rat): S865‑p, HSL iso2 (rat): S565‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
isoproterenol no change compared to control
rapamycin isoproterenol no change compared to control

S650-p - HSL (mouse)
Orthologous residues
HSL (human): S950‑p, HSL iso2 (human): S649‑p, HSL (mouse): S650‑p, HSL iso3 (mouse): S963‑p, HSL (rat): S959‑p, HSL iso2 (rat): S659‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse

S651-p - HSL (mouse)
Orthologous residues
HSL (human): S951‑p, HSL iso2 (human): S650‑p, HSL (mouse): S651‑p, HSL iso3 (mouse): S964‑p, HSL (rat): S960‑p, HSL iso2 (rat): S660‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
isoproterenol increase
rapamycin isoproterenol no effect upon treatment-induced increase

S2481-p - mTOR (mouse)
Orthologous residues
mTOR (human): S2481‑p, mTOR (mouse): S2481‑p, mTOR (rat): S2481‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
rapamycin insulin inhibit treatment-induced increase

T412-p - p70S6K (mouse)
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
rapamycin insulin inhibit treatment-induced increase

S96-p - PKAR2A (mouse)
Orthologous residues
PKAR2A (human): S99‑p, PKAR2A (mouse): S96‑p, PKAR2A (rat): S97‑p, PKAR2A (cow): S96‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
isoproterenol increase
rapamycin isoproterenol no effect upon treatment-induced increase

S112-p - PKAR2B (mouse)
Orthologous residues
PKAR2B (human): S114‑p, PKAR2B (mouse): S112‑p, PKAR2B (rat): S112‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
isoproterenol increase
rapamycin isoproterenol no effect upon treatment-induced increase


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