Curated Information
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Curated Information Page
PubMed Id: 21045017 
Wang P, et al. (2011) Serine phosphorylation of NPM-ALK, which is dependent on the auto-activation of the kinase activation loop, contributes to its oncogenic potential. Carcinogenesis 32, 146-53 21045017
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T202-p - ERK1 (human)
Orthologous residues
ERK1 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  GP293 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
NPM-ALK (human) increase

Y204-p - ERK1 (human)
Orthologous residues
ERK1 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  GP293 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
NPM-ALK (human) increase

T185-p - ERK2 (human)
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  GP293 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
NPM-ALK (human) increase

Y187-p - ERK2 (human)
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  GP293 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
NPM-ALK (human) increase

T183-p - JNK1 (human)
Orthologous residues
JNK1 (human): T183‑p, JNK1 iso2 (human): T183‑p, JNK1 iso3 (human): T183‑p, JNK1 (mouse): T183‑p, JNK1 (rat): T183‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  GP293 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
NPM-ALK (human) increase

Y185-p - JNK1 (human)
Orthologous residues
JNK1 (human): Y185‑p, JNK1 iso2 (human): Y185‑p, JNK1 iso3 (human): Y185‑p, JNK1 (mouse): Y185‑p, JNK1 (rat): Y185‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  GP293 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
NPM-ALK (human) increase

S135-p - NPM-ALK (human)
Orthologous residues
NPM‑ALK (human): S135‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  lymphoma, anaplastic large cell lymphoma, T cell lymphoma
 Relevant cell lines - cell types - tissues:  GP293 (fibroblast), Karpas-299 (T lymphocyte), SU-DHL1 (T lymphocyte), SUP-M2 (T lymphocyte)
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PD98059 decrease
SB203580 decrease
U0126 decrease
SP600125 decrease
Downstream Regulation
 Effect of modification (function):  phosphorylation
 Effect of modification (process):  carcinogenesis, altered, cell growth, altered
 Comments:  mutation of this site reduces the phosphorylation of JNK, ERK1/2, Stat6; required for phosphorylation of residues S164 and S497

Y138-p - NPM-ALK (human)
Orthologous residues
NPM‑ALK (human): Y138‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  GP293 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human

Y152-p - NPM-ALK (human)
Orthologous residues
NPM‑ALK (human): Y152‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  GP293 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human

Y156-p - NPM-ALK (human)
Orthologous residues
NPM‑ALK (human): Y156‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  GP293 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human

S164-p - NPM-ALK (human)
Orthologous residues
NPM‑ALK (human): S164‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  lymphoma, anaplastic large cell lymphoma, T cell lymphoma
 Relevant cell lines - cell types - tissues:  GP293 (fibroblast), Karpas-299 (T lymphocyte), SU-DHL1 (T lymphocyte), SUP-M2 (T lymphocyte)
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
D4476 decrease
GSK-3 inhibitor IX decrease
Downstream Regulation
 Effect of modification (function):  phosphorylation
 Effect of modification (process):  carcinogenesis, altered, cell growth, altered
 Comments:  mutation of this site reduces the phosphorylation of JNK, ERK1/2, Stat6

Y191-p - NPM-ALK (human)
Orthologous residues
NPM‑ALK (human): Y191‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  GP293 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human

Y338-p - NPM-ALK (human)
Orthologous residues
NPM‑ALK (human): Y338‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  GP293 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human

Y342-p - NPM-ALK (human)
Orthologous residues
NPM‑ALK (human): Y342‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  GP293 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human

Y343-p - NPM-ALK (human)
Orthologous residues
NPM‑ALK (human): Y343‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  GP293 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human

Y419-p - NPM-ALK (human)
Orthologous residues
NPM‑ALK (human): Y419‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  GP293 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human

S497-p - NPM-ALK (human)
Orthologous residues
NPM‑ALK (human): S497‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  lymphoma, anaplastic large cell lymphoma, T cell lymphoma
 Relevant cell lines - cell types - tissues:  GP293 (fibroblast), Karpas-299 (T lymphocyte), SU-DHL1 (T lymphocyte), SUP-M2 (T lymphocyte)
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PD98059 decrease
SB203580 decrease
U0126 decrease
SP600125 decrease
Downstream Regulation
 Effect of modification (function):  phosphorylation
 Effect of modification (process):  carcinogenesis, altered, cell growth, altered
 Comments:  mutation of this site reduces the phosphorylation of JNK, ERK1/2, Stat6

Y567-p - NPM-ALK (human)
Orthologous residues
NPM‑ALK (human): Y567‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  GP293 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human

Y644-p - NPM-ALK (human)
Orthologous residues
NPM‑ALK (human): Y644‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  GP293 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human

Y664-p - NPM-ALK (human)
Orthologous residues
NPM‑ALK (human): Y664‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  GP293 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human

Y641-p - STAT6 (human)
Orthologous residues
STAT6 (human): Y641‑p, STAT6 (mouse): Y641‑p, STAT6 (rat): Y641‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  GP293 (fibroblast), Jurkat (T lymphocyte)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
NPM-ALK (human) increase


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