Curated Information
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Curated Information Page
PubMed Id: 21081666 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Greco TM, Yu F, Guise AJ, Cristea IM (2011) Nuclear import of histone deacetylase 5 by requisite nuclear localization signal phosphorylation. Mol Cell Proteomics 10, M110.004317 21081666
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

S3-p - HDAC5 (human)
Orthologous residues
HDAC5 (human): S3‑p, HDAC5 (mouse): S3‑p, HDAC5 (rat):
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S7-p - HDAC5 (human)
Orthologous residues
HDAC5 (human): S7‑p, HDAC5 (mouse): S7‑p, HDAC5 (rat):
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S53-p - HDAC5 (human)
Orthologous residues
HDAC5 (human): S53‑p, HDAC5 (mouse): S53‑p, HDAC5 (rat):
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S55-p - HDAC5 (human)
Orthologous residues
HDAC5 (human): S55‑p, HDAC5 (mouse): S55‑p, HDAC5 (rat):
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S66-p - HDAC5 (human)
Orthologous residues
HDAC5 (human): S66‑p, HDAC5 (mouse): P66‑p, HDAC5 (rat):
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S206-p - HDAC5 (human)
Orthologous residues
HDAC5 (human): S206‑p, HDAC5 (mouse): S197‑p, HDAC5 (rat): S30‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

T234-p - HDAC5 (human)
Orthologous residues
HDAC5 (human): T234‑p, HDAC5 (mouse): T225‑p, HDAC5 (rat): T58‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S259-p - HDAC5 (human)
Orthologous residues
HDAC5 (human): S259‑p, HDAC5 (mouse): S250‑p, HDAC5 (rat): S83‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, western blotting
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  293 (epithelial), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  enzymatic activity, inhibited, molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PKD3 (human) Disrupts electrophoretic visualization
14-3-3 epsilon (human) Induces electrophoretic visualization
PKD2 (human) Disrupts electrophoretic visualization
N-CoR1 (human) Induces electrophoretic visualization
GPS2 (human) Disrupts electrophoretic visualization
TBL1XR1 (human) Disrupts electrophoretic visualization
PKD1 (human) Disrupts electrophoretic visualization
HDAC3 (human) Disrupts electrophoretic visualization
TBL1X (human) Disrupts electrophoretic visualization
SMRT (human) Disrupts electrophoretic visualization
 Comments:  S259/498A HDAC5 mutant exhibits a significant increase in deacetylation activity

S278-p - HDAC5 (human)
Orthologous residues
HDAC5 (human): S278‑p, HDAC5 (mouse): S269‑p, HDAC5 (rat): S102‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, western blotting
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  293 (epithelial), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced, molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
TBL1X (human) Induces electrophoretic visualization
SMRT (human) Induces electrophoretic visualization
GPS2 (human) Induces electrophoretic visualization
HDAC3 (human) Induces electrophoretic visualization
TBL1XR1 (human) Induces electrophoretic visualization
N-CoR1 (human) Disrupts electrophoretic visualization
 Comments:  S278/279A mutant shows a slight decrease in the deacetylation activity

S279-p - HDAC5 (human)
Orthologous residues
HDAC5 (human): S279‑p, HDAC5 (mouse): S270‑p, HDAC5 (rat): S103‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, western blotting
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  293 (epithelial), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced, intracellular localization, molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SMRT (human) Induces electrophoretic visualization
N-CoR1 (human) Disrupts electrophoretic visualization
GPS2 (human) Induces electrophoretic visualization
HDAC3 (human) Induces electrophoretic visualization
TBL1X (human) Induces electrophoretic visualization
TBL1XR1 (human) Induces electrophoretic visualization
 Comments:  S278/279A mutant shows a slight decrease in the deacetylation activity

S368-p - HDAC5 (human)
Orthologous residues
HDAC5 (human): S368‑p, HDAC5 (mouse): S359‑p, HDAC5 (rat): S192‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S498-p - HDAC5 (human)
Orthologous residues
HDAC5 (human): S498‑p, HDAC5 (mouse): S488‑p, HDAC5 (rat): S322‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, western blotting
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  293 (epithelial), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  enzymatic activity, inhibited, molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 epsilon (human) Induces electrophoretic visualization
HDAC3 (human) Disrupts electrophoretic visualization
TBL1XR1 (human) Disrupts electrophoretic visualization
GPS2 (human) Disrupts electrophoretic visualization
TBL1X (human) Disrupts electrophoretic visualization
PKD1 (human) Disrupts electrophoretic visualization
PKD3 (human) Disrupts electrophoretic visualization
SMRT (human) Disrupts electrophoretic visualization
N-CoR1 (human) Induces electrophoretic visualization
PKD2 (human) Disrupts electrophoretic visualization
 Comments:  S259/498A HDAC5 mutant exhibits a significant increase in deacetylation activity

S611-p - HDAC5 (human)
Orthologous residues
HDAC5 (human): S611‑p, HDAC5 (mouse): S600‑p, HDAC5 (rat): S433‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, western blotting
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  293 (epithelial), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human

S661-p - HDAC5 (human)
Orthologous residues
HDAC5 (human): S661‑p, HDAC5 (mouse): S650‑p, HDAC5 (rat): S483‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S671-p - HDAC5 (human)
Orthologous residues
HDAC5 (human): S671‑p, HDAC5 (mouse): S660‑p, HDAC5 (rat): S493‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S755-p - HDAC5 (human)
Orthologous residues
HDAC5 (human): S755‑p, HDAC5 (mouse): S746‑p, HDAC5 (rat): S579‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, western blotting
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  293 (epithelial), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human

S1108-p - HDAC5 (human)
Orthologous residues
HDAC5 (human): S1108‑p, HDAC5 (mouse): S1099‑p, HDAC5 (rat): S932‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, western blotting
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  293 (epithelial), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human


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