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Orthologous residues
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MAD2L1 (human): S195‑p, MAD2L1 (mouse): S195‑p
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Characterization
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Methods used to characterize site in vivo:
[32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site
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Relevant cell lines - cell types - tissues:
HeLa (cervical)
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Cellular systems studied:
cell lines
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Species studied:
human
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Downstream Regulation
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Effect of modification (function):
inhibition, molecular association, regulation, protein conformation
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Effect of modification (process):
cell cycle regulation
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Modification regulates interactions with:
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Interacting molecule
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Interacting domains
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Effect
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Consequences (function)
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Consequences (process)
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Detection assays
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Cdc20 (human)
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Disrupts
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co-immunoprecipitation, in vitro
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Comments:
MAD2L1 S195D mutant bound to Mad1 with lower affinity in vitro but higher in vivo, as compared to MAD2L1-wt; phosphomimicking 3D mutant (S170, S185, S195) prevented Mad2 conformational transition in vitro
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