Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Curated Information Page
PubMed Id: 21041666 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Kim S, et al. (2010) Phosphorylation of the spindle checkpoint protein Mad2 regulates its conformational transition. Proc Natl Acad Sci U S A 107, 19772-7 21041666
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

S170-p - MAD2L1 (human)
Orthologous residues
MAD2L1 (human): S170‑p, MAD2L1 (mouse): S170‑p
Downstream Regulation
 Effect of modification (function):  protein conformation
 Comments:  phosphomimicking 3D mutant (S170, S185, S195) prevented Mad2 conformational transition in vitro

S178-p - MAD2L1 (human)
Orthologous residues
MAD2L1 (human): S178‑p, MAD2L1 (mouse): C178‑p

S185-p - MAD2L1 (human)
Orthologous residues
MAD2L1 (human): S185‑p, MAD2L1 (mouse): S185‑p
Downstream Regulation
 Effect of modification (function):  protein conformation
 Comments:  phosphomimicking 3D mutant (S170, S185, S195) prevented Mad2 conformational transition in vitro

S195-p - MAD2L1 (human)
Orthologous residues
MAD2L1 (human): S195‑p, MAD2L1 (mouse): S195‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  activity, inhibited, molecular association, regulation, protein conformation
 Effect of modification (process):  cell cycle regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Cdc20 (human) Disrupts co-immunoprecipitation, in vitro
 Comments:  MAD2L1 S195D mutant bound to Mad1 with lower affinity in vitro but higher in vivo, as compared to MAD2L1-wt; phosphomimicking 3D mutant (S170, S185, S195) prevented Mad2 conformational transition in vitro


Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.