Curated Information
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Curated Information Page
PubMed Id: 21059642 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Guo H, Friedman AD (2011) Phosphorylation of RUNX1 by cyclin-dependent kinase reduces direct interaction with HDAC1 and HDAC3. J Biol Chem 286, 208-15 21059642
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S48-p - AML1 iso8 (human) ▲
Orthologous residues
AML1 (human): S21‑p, AML1 iso2 (human): S21‑p, AML1 iso8 (human): S48‑p, AML1 (mouse): S21‑p, AML1 (rat): S21‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  cell growth, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
HDAC3 (human) Disrupts co-immunoprecipitation
HDAC1 (human) Disrupts co-immunoprecipitation
 Comments:  increases marrow progenitor proliferation

S303-p - AML1 iso8 (human) ▲
Orthologous residues
AML1 (human): S276‑p, AML1 iso2 (human): S276‑p, AML1 iso8 (human): S303‑p, AML1 (mouse): S276‑p, AML1 (rat): S275‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  cell growth, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
HDAC3 (human) Disrupts co-immunoprecipitation
HDAC1 (human) Disrupts co-immunoprecipitation
 Comments:  increases marrow progenitor proliferation

S424-p - AML1 iso8 (human) ▲
Orthologous residues
AML1 (human): S397‑p, AML1 iso2 (human): S397‑p, AML1 iso8 (human): S424‑p, AML1 (mouse): S396‑p, AML1 (rat): S395‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE CDK1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
roscovitine decrease
U0126 no change compared to control
LY294002 no change compared to control
PP2 no change compared to control
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  cell growth, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
HDAC1 (human) Disrupts co-immunoprecipitation
HDAC3 (human) Disrupts co-immunoprecipitation
 Comments:  increases marrow progenitor proliferation


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