Curated Information
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Curated Information Page
PubMed Id: 10980595 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Mérienne K, et al. (2000) Activation of RSK by UV-light: phosphorylation dynamics and involvement of the MAPK pathway. Oncogene 19, 4221-9 10980595
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S227-p - RSK2 (human) ▲
Orthologous residues
RSK2 (human): S227‑p, RSK2 (mouse): S227‑p, RSK2 (rat): S227‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast), CLS (fibroblast), COS (fibroblast), CV1 (fibroblast), fibroblast
 Cellular systems studied:  cell lines, primary cultured cells
 Species studied:  human, monkey, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
EGF increase
phorbol ester increase
arsenite increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced

T365-p - RSK2 (human) ▲
Orthologous residues
RSK2 (human): T365‑p, RSK2 (mouse): T365‑p, RSK2 (rat): T365‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast), CLS (fibroblast), COS (fibroblast), CV1 (fibroblast), fibroblast
 Cellular systems studied:  cell lines, primary cultured cells
 Species studied:  human, monkey, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
EGF increase
phorbol ester increase
arsenite increase

S369-p - RSK2 (human) ▲
Orthologous residues
RSK2 (human): S369‑p, RSK2 (mouse): S369‑p, RSK2 (rat): S369‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast), CLS (fibroblast), COS (fibroblast), CV1 (fibroblast), fibroblast
 Cellular systems studied:  cell lines, primary cultured cells
 Species studied:  human, monkey, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
EGF increase
phorbol ester increase
arsenite increase

S386-p - RSK2 (human) ▲
Orthologous residues
RSK2 (human): S386‑p, RSK2 (mouse): S386‑p, RSK2 (rat): S386‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast), CLS (fibroblast), COS (fibroblast), CV1 (fibroblast), fibroblast
 Cellular systems studied:  cell lines, primary cultured cells
 Species studied:  human, monkey, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
EGF increase
phorbol ester increase
arsenite increase

T577-p - RSK2 (human) ▲
Orthologous residues
RSK2 (human): T577‑p, RSK2 (mouse): T577‑p, RSK2 (rat): T577‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast), CLS (fibroblast), COS (fibroblast), CV1 (fibroblast), fibroblast
 Cellular systems studied:  cell lines, primary cultured cells
 Species studied:  human, monkey, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol ester increase
arsenite increase
EGF increase
PD98059 EGF inhibit treatment-induced increase
SB203580 EGF no effect upon treatment-induced increase
UV increase
PD98059 UV inhibit treatment-induced increase
SB203580 UV inhibit treatment-induced increase COS cells
SB203580 UV no effect upon treatment-induced increase normal human fibroblasts
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced


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