Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Curated Information Page
PubMed Id: 11970864 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Kornhauser JM, et al. (2002) CREB transcriptional activity in neurons is regulated by multiple, calcium-specific phosphorylation events. Neuron 34, 221-33 11970864
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

S133-p - CREB (rat)
Orthologous residues
CREB (human): S133‑p, CREB iso2 (human): S119‑p, CREB (mouse): S133‑p, CREB (rat): S133‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody
 Relevant cell lines - cell types - tissues:  'neuron, cortical'-brain
 Cellular systems studied:  cell lines, primary cultured cells, tissue
 Species studied:  rat
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKACA (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PTZ increase
BDNF increase
depolarization increase The kinetics of phosphorylation of S133, S142 and S143 may differ from each other.
cAMP analog increase
glutamate increase
KN-62 glutamate no effect upon treatment-induced increase
PD98059 glutamate inhibit treatment-induced increase
Downstream Regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
CBP (rat) KIX Induces co-immunoprecipitation, in vitro
 Comments:  The binding of phospho-S133 to CBP via its KIX domain is inhibited by the phosphorylation of S142 and S143.

S142-p - CREB (rat)
Orthologous residues
CREB (human): S142‑p, CREB iso2 (human): S128‑p, CREB (mouse): S142‑p, CREB (rat): S142‑p
Characterization
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2A (rat)
KINASE CK2A1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PTZ increase
depolarization increase
BDNF no change compared to control
glutamate increase
KN-62 glutamate inhibit treatment-induced increase
PD98059 glutamate no effect upon treatment-induced increase
Downstream Regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
CBP (rat) KIX Disrupts co-immunoprecipitation, in vitro

S143-p - CREB (rat)
Orthologous residues
CREB (human): S143‑p, CREB iso2 (human): S129‑p, CREB (mouse): S143‑p, CREB (rat): S143‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody
 Relevant cell lines - cell types - tissues:  'neuron, cortical'-brain
 Cellular systems studied:  cell lines, primary cultured cells, tissue
 Species studied:  rat
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK2A1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
glutamate increase
PTZ increase
BDNF no change compared to control
depolarization increase
cAMP analog no change compared to control
Downstream Regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
CBP (rat) KIX Disrupts co-immunoprecipitation, in vitro


Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.