Curated Information

Javascript is not enabled on this browser. This site will not work properly without Javascript.

Home

Curated Information Page

PubMed Id: 9405465

This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus^®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus^®, click here.

Mammen AL, Kameyama K, Roche KW, Huganir RL (1997) Phosphorylation of the alpha-amino-3-hydroxy-5-methylisoxazole4-propionic acid receptor GluR1 subunit by calcium/calmodulin-dependent kinase II. J Biol Chem 272, 32528-33 9405465

Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.

S849-p - GluR1 (rat)

▲

Orthologous residues

GluR1 (human): S849‑p, GluR1 (mouse): S849‑p, GluR1 (rat): S849‑p

Characterization

Methods used to characterize site in vivo: [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphopeptide mapping

Relevant cell lines - cell types - tissues: 'brain, hippocampus', 293 (epithelial), QT-6 (fibroblast) [CaMK2-alpha (human)], QT-6 (fibroblast) [GluR1 (rat)]

Cellular systems studied: cell lines, primary cells

Species studied: human, quail, rat

Enzymes shown to modify site in vitro:

Type	Enzyme
KINASE	CaMK2-alpha (rat)

Upstream Regulation

Potential in vivo enzymes for site:

Type	Enzyme	Evidence	Notes
KINASE	CaMK2-alpha (rat)	pharmacological inhibitor of upstream enzyme, transfection of constitutively active enzyme, pharmacological activator of upstream enzyme

Treatments, proteins and their effect on site modification:

Treatments	Referenced Treatments	Effect
phorbol ester		increase
KN-62		decrease
A23187		increase
KN-62	A23187	inhibit treatment-induced increase

S863-p - GluR1 (rat)

▲

Orthologous residues

GluR1 (human): S863‑p, GluR1 (mouse): S863‑p, GluR1 (rat): S863‑p

Characterization

Methods used to characterize site in vivo: 2D analysis, [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphopeptide mapping

Relevant cell lines - cell types - tissues: 'brain, hippocampus', 293 (epithelial), QT-6 (fibroblast) [GluR1 (rat)]

Cellular systems studied: cell lines, primary cells

Species studied: human, quail, rat

Upstream Regulation

Treatments, proteins and their effect on site modification:

Treatments	Referenced Treatments	Manipulated Protein	Referenced Protein	Effect	Notes
forskolin				increase
forskolin, IBMX				increase

Home \| Curator Login	With enhanced literature mining using Linguamatics I2E		Produced by 3rd Millennium \| Design by Digizyme
			©2003-2013 Cell Signaling Technology, Inc.