Curated Information
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Curated Information Page
PubMed Id: 11146653 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Yang DQ, Kastan MB (2000) Participation of ATM in insulin signalling through phosphorylation of eIF-4E-binding protein 1. Nat Cell Biol 2, 893-8 11146653
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S111-p - 4E-BP1 (rat) ▲
Orthologous residues
4E‑BP1 (human): S112‑p, 4E‑BP1 (mouse): S111‑p, 4E‑BP1 (rat): S111‑p, 4E‑BP1 (fruit fly): ‑
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293T (epithelial), GM0637 (fibroblast), GM9607 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ATM (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) transfection of inactive enzyme, modification site within consensus motif, transfection of wild-type enzyme, genetic knockout/knockin of upstream enzyme, phosphopeptide analysis, pharmacological activator of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
rapamycin insulin no effect upon treatment-induced increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
eIF4E (human) Disrupts co-immunoprecipitation


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