Curated Information
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PubMed Id: 11500362 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Wang X, et al. (2001) Eukaryotic initiation factor 2B: identification of multiple phosphorylation sites in the epsilon-subunit and their functions in vivo. EMBO J 20, 4349-59 11500362
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S466-p - eIF2B-epsilon (human) ▲
Orthologous residues
eIF2B‑epsilon (human): S466‑p, eIF2B‑epsilon (mouse): S462‑p, eIF2B‑epsilon (rat): S461‑p, eIF2B‑epsilon (rabbit): S466‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S469-p - eIF2B-epsilon (human) ▲
Orthologous residues
eIF2B‑epsilon (human): S469‑p, eIF2B‑epsilon (mouse): S465‑p, eIF2B‑epsilon (rat): S464‑p, eIF2B‑epsilon (rabbit): S469‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S717-p - eIF2B-epsilon (human) ▲
Orthologous residues
eIF2B‑epsilon (human): S717‑p, eIF2B‑epsilon (mouse): S713‑p, eIF2B‑epsilon (rat): S712‑p, eIF2B‑epsilon (rabbit): S717‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S718-p - eIF2B-epsilon (human) ▲
Orthologous residues
eIF2B‑epsilon (human): S718‑p, eIF2B‑epsilon (mouse): S714‑p, eIF2B‑epsilon (rat): S713‑p, eIF2B‑epsilon (rabbit): S718‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S461-p - eIF2B-epsilon (rat) ▲
Orthologous residues
eIF2B‑epsilon (human): S466‑p, eIF2B‑epsilon (mouse): S462‑p, eIF2B‑epsilon (rat): S461‑p, eIF2B‑epsilon (rabbit): S466‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S464-p - eIF2B-epsilon (rat) ▲
Orthologous residues
eIF2B‑epsilon (human): S469‑p, eIF2B‑epsilon (mouse): S465‑p, eIF2B‑epsilon (rat): S464‑p, eIF2B‑epsilon (rabbit): S469‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE CK1-A (human)

S535-p - eIF2B-epsilon (rat) ▲
Orthologous residues
eIF2B‑epsilon (human): S540‑p, eIF2B‑epsilon (mouse): S536‑p, eIF2B‑epsilon (rat): S535‑p, eIF2B‑epsilon (rabbit): S540‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, peptide sequencing, phospho-antibody, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE GSK3A (rat)

S539-p - eIF2B-epsilon (rat) ▲
Orthologous residues
eIF2B‑epsilon (human): S544‑p, eIF2B‑epsilon (mouse): S540‑p, eIF2B‑epsilon (rat): S539‑p, eIF2B‑epsilon (rabbit): S544‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site, peptide sequencing, phospho-antibody, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial), Sf21
 Cellular systems studied:  cell lines
 Species studied:  human, insect

S712-p - eIF2B-epsilon (rat) ▲
Orthologous residues
eIF2B‑epsilon (human): S717‑p, eIF2B‑epsilon (mouse): S713‑p, eIF2B‑epsilon (rat): S712‑p, eIF2B‑epsilon (rabbit): S717‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE CK2-A1 (rat)
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
eIF2-alpha (human) Induces co-immunoprecipitation

S713-p - eIF2B-epsilon (rat) ▲
Orthologous residues
eIF2B‑epsilon (human): S718‑p, eIF2B‑epsilon (mouse): S714‑p, eIF2B‑epsilon (rat): S713‑p, eIF2B‑epsilon (rabbit): S718‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE CK2-A1 (rat)
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
eIF2-alpha (human) Induces co-immunoprecipitation


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