|
Orthologous residues
|
|
STEP (human): S245‑p, STEP (mouse): S221‑p, STEP (rat): S49‑p
|
|
Characterization
|
|
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling, immunoprecipitation, phosphoamino acid analysis, phosphopeptide mapping
|
|
Relevant cell lines - cell types - tissues:
'brain, striatum'
|
|
Cellular systems studied:
tissue
|
|
Species studied:
rat
|
|
Enzymes shown to modify site in vitro:
|
|
|
|
Upstream Regulation
|
|
Potential in vivo enzymes for site:
|
|
Type
|
Enzyme
|
Evidence
|
Notes
|
|
KINASE
|
PKACa (rat)
|
phospho-antibody, pharmacological inhibitor of upstream enzyme
|
|
|
|
Treatments, proteins and their effect on site modification:
|
|
Treatments
|
Referenced Treatments
|
Manipulated Protein
|
Referenced Protein
|
Effect
|
Notes
|
|
SKF81297
|
|
|
|
increase
|
|
|
H-89
|
SKF81297
|
|
|
inhibit treatment-induced increase
|
|
|
dopamine
|
SKF81297
|
|
|
augment treatment-induced increase
|
|
|
SCH 23390
|
SKF81297
|
|
|
inhibit treatment-induced increase
|
|
|
dopamine
|
|
|
|
increase
|
|
|
quinpirole
|
dopamine
|
|
|
no effect upon treatment-induced increase
|
|
|
SCH 23390
|
dopamine
|
|
|
inhibit treatment-induced increase
|
|
|
H-89
|
|
|
|
no change compared to control
|
|
|
