Curated Information
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PubMed Id: 20966048 
Wu S, et al. (2010) Dynamic regulation of the PR-Set7 histone methyltransferase is required for normal cell cycle progression. Genes Dev 24, 2531-42 20966048
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
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S100-p - SETD8 (human)
Orthologous residues
SETD8 (human): S100‑p, SETD8 (mouse): S57‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  in mitotic cells
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CDK1 (human) pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
CGP74514A decrease
D4476 no change compared to control
siRNA decrease shRNA cyclinB
Downstream Regulation
 Effect of modification (function):  intracellular localization, protein stabilization, ubiquitination
 Effect of modification (process):  cell cycle regulation
 Comments:  phosphorylation of this site results in the removal of SETD8 from mitotic chromosomes; inhibits SETD8 ubiquitination


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