Curated Information
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Curated Information Page
PubMed Id: 20450893 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Barisic S, Schmidt C, Walczak H, Kulms D (2010) Tyrosine phosphatase inhibition triggers sustained canonical serine-dependent NFkappaB activation via Src-dependent blockade of PP2A. Biochem Pharmacol 80, 439-47 20450893
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S32-p - IkB-alpha (human)
Orthologous residues
IkB‑alpha (human): S32‑p, IkB‑alpha (mouse): S32‑p, IkB‑alpha (rat): S32‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  cervical cancer, cervical squamous cell carcinoma
 Relevant cell lines - cell types - tissues:  KB (squamous)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IL-1b no change compared to control
MG132, IL-1b increase
vanadate, IL-1b no change compared to control

S36-p - IkB-alpha (human)
Orthologous residues
IkB‑alpha (human): S36‑p, IkB‑alpha (mouse): S36‑p, IkB‑alpha (rat): S36‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  cervical cancer, cervical squamous cell carcinoma
 Relevant cell lines - cell types - tissues:  KB (squamous)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IL-1b no change compared to control
MG132, IL-1b increase
vanadate, IL-1b no change compared to control

S177-p - IKKB (human)
Orthologous residues
IKKB (human): S177‑p, IKKB (mouse): S177‑p, IKKB (rat): S177‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  cervical cancer, cervical squamous cell carcinoma
 Relevant cell lines - cell types - tissues:  KB (squamous)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IL-1b increase
MG132 IL-1b no effect upon treatment-induced increase
vanadate IL-1b inhibit treatment-induced increase
LY294002 IL-1b, vanadate augment treatment-induced increase
PD98059 IL-1b, vanadate no effect upon treatment-induced increase
SP600125 IL-1b, vanadate no effect upon treatment-induced increase
Akt-I-1 IL-1b, vanadate no effect upon treatment-induced increase
calyculin A IL-1b augment treatment-induced increase
calyculin A, IL-1b PPP2CA (human) decrease PPP2CA siRNA increases
IL-1b, vanadate Src (human) augment treatment-induced increase constitutively active Src
IL-1b Src (human) no effect upon treatment-induced increase Src constitutively active
herbimycin A IL-1b, vanadate inhibit treatment-induced increase

S181-p - IKKB (human)
Orthologous residues
IKKB (human): S181‑p, IKKB (mouse): S181‑p, IKKB (rat): S181‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  cervical cancer, cervical squamous cell carcinoma
 Relevant cell lines - cell types - tissues:  KB (squamous)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IL-1b increase
MG132 IL-1b no effect upon treatment-induced increase
vanadate IL-1b inhibit treatment-induced increase
LY294002 IL-1b, vanadate augment treatment-induced increase
PD98059 IL-1b, vanadate no effect upon treatment-induced increase
SP600125 IL-1b, vanadate no effect upon treatment-induced increase
Akt-I-1 IL-1b, vanadate no effect upon treatment-induced increase
calyculin A IL-1b augment treatment-induced increase
calyculin A, IL-1b PPP2CA (human) decrease PPP2CA siRNA increases
IL-1b, vanadate Src (human) augment treatment-induced increase constitutively active Src
IL-1b Src (human) no effect upon treatment-induced increase Src constitutively active
herbimycin A IL-1b, vanadate inhibit treatment-induced increase

Y188-p - IKKB (human)
Orthologous residues
IKKB (human): Y188‑p, IKKB (mouse): Y188‑p, IKKB (rat): Y188‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  cervical cancer, cervical squamous cell carcinoma
 Relevant cell lines - cell types - tissues:  KB (squamous)
 Cellular systems studied:  cell lines
 Species studied:  human

Y199-p - IKKB (human)
Orthologous residues
IKKB (human): Y199‑p, IKKB (mouse): Y199‑p, IKKB (rat): Y199‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  cervical cancer, cervical squamous cell carcinoma
 Relevant cell lines - cell types - tissues:  KB (squamous)
 Cellular systems studied:  cell lines
 Species studied:  human

Y307-p - PPP2CA (human)
Orthologous residues
PPP2CA (human): Y307‑p, PPP2CA (mouse): Y307‑p, PPP2CA (rat): Y307‑p, PPP2CA (pig): Y307‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
 Disease tissue studied:  cervical cancer, cervical squamous cell carcinoma
 Relevant cell lines - cell types - tissues:  KB (squamous)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Src (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Src (human) transfection of inactive enzyme, phospho-antibody, transfection of constitutively active enzyme, co-immunoprecipitation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IL-1b no change compared to control
vanadate, IL-1b increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, inhibited

Y419-p - Src (human)
Orthologous residues
Src (human): Y419‑p, Src (mouse): Y424‑p, Src iso2 (mouse): Y418‑p, Src (rat): Y419‑p, Src (chicken): Y416‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  cervical cancer, cervical squamous cell carcinoma
 Relevant cell lines - cell types - tissues:  KB (squamous)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IL-1b no change compared to control
vanadate, IL-1b increase


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