Curated Information
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Curated Information Page
PubMed Id: 20713355 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Guo X, et al. (2010) Interdependent phosphorylation within the kinase domain T-loop Regulates CHK2 activity. J Biol Chem 285, 33348-57 20713355
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S33-p - Chk2 (human)
Orthologous residues
Chk2 (human): S33‑p, Chk2 iso12 (human): S33‑p, Chk2 (mouse): S36‑p, Chk2 (rat): S35‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase

S35-p - Chk2 (human)
Orthologous residues
Chk2 (human): S35‑p, Chk2 iso12 (human): S35‑p, Chk2 (mouse): S42‑p, Chk2 (rat): S41‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase

T68-p - Chk2 (human)
Orthologous residues
Chk2 (human): T68‑p, Chk2 iso12 (human): T68‑p, Chk2 (mouse): T77‑p, Chk2 (rat): T76‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase

S372-p - Chk2 (human)
Orthologous residues
Chk2 (human): S372‑p, Chk2 iso12 (human): S343‑p, Chk2 (mouse): S376‑p, Chk2 (rat): S375‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation no change compared to control
Downstream Regulation
 Effect of modification (function):  intracellular localization
 Comments:  chromatin targeting of S372, S379, T389, singly and T383/389, T378/S379

T378-p - Chk2 (human)
Orthologous residues
Chk2 (human): T378‑p, Chk2 iso12 (human): T349‑p, Chk2 (mouse): T382‑p, Chk2 (rat): T381‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation decrease
Downstream Regulation
 Effect of modification (function):  intracellular localization
 Comments:  chromatin targeting of S372, S379, T389, singly and T383/389, T378/S379

S379-p - Chk2 (human)
Orthologous residues
Chk2 (human): S379‑p, Chk2 iso12 (human): S350‑p, Chk2 (mouse): S383‑p, Chk2 (rat): S382‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced, intracellular localization, ubiquitination
 Comments:  chromatin targeting of S372, S379, T389, singly and T383/389, T378/S379

T383-p - Chk2 (human)
Orthologous residues
Chk2 (human): T383‑p, Chk2 iso12 (human): T354‑p, Chk2 (mouse): T387‑p, Chk2 (rat): T386‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation no change compared to control
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced, intracellular localization, ubiquitination
 Comments:  chromatin targeting of S372, S379, T389, singly and T383/389, T378/S379

T387-p - Chk2 (human)
Orthologous residues
Chk2 (human): T387‑p, Chk2 iso12 (human): T358‑p, Chk2 (mouse): T391‑p, Chk2 (rat): T390‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation no change compared to control
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced, intracellular localization
 Comments:  chromatin targeting of S372, S379, T389, singly and T383/389, T378/S379

T389-p - Chk2 (human)
Orthologous residues
Chk2 (human): T389‑p, Chk2 iso12 (human): T360‑p, Chk2 (mouse): T393‑p, Chk2 (rat): T392‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase
Downstream Regulation
 Effect of modification (function):  intracellular localization, phosphorylation
 Comments:  chromatin targeting of S372, S379, T389, singly and T383/389, T378/S379; prerequisite for T387 phosphorylation

Y390-p - Chk2 (human)
Orthologous residues
Chk2 (human): Y390‑p, Chk2 iso12 (human): Y361‑p, Chk2 (mouse): Y394‑p, Chk2 (rat): Y393‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation decrease
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced, intracellular localization
 Comments:  chromatin targeting of S372, S379, T389, singly and T383/389, T378/S379

S516-p - Chk2 (human)
Orthologous residues
Chk2 (human): S516‑p, Chk2 iso12 (human): S487‑p, Chk2 (mouse): S520‑p, Chk2 (rat): S519‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase


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