Curated Information
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Curated Information Page
PubMed Id: 20647423 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Bungard D, et al. (2010) Signaling kinase AMPK activates stress-promoted transcription via histone H2B phosphorylation. Science 329, 1201-5 20647423
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S37-p - H2B (human)
Orthologous residues
H2B (human): S37‑p, H2B (mouse): S37‑p, H2B (rat): S37‑p, H2B (cow): S36‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE AMPKA1 (mouse)

S79-p - ACC1 (mouse)
Orthologous residues
ACC1 (human): S80‑p, ACC1 iso4 (human): S117‑p, ACC1 (mouse): S79‑p, ACC1 iso2 (mouse): S117‑p, ACC1 (rat): S79‑p, ACC1 iso2 (rat): S79‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
ionizing radiation increase
camptothecin increase
adriamycin increase
MNNG increase
H2O2 increase
2-deoxyglucose increase
AICAR increase
phenformin increase
2-deoxyglucose increase
AICAR increase

T183-p - AMPKA1 (mouse)
Orthologous residues
AMPKA1 (human): T183‑p, AMPKA1 (mouse): T183‑p, AMPKA1 (rat): T183‑p, AMPKA1 (fruit fly): T184‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
ionizing radiation increase
camptothecin increase
adriamycin increase
MNNG increase
H2O2 increase
2-deoxyglucose increase
AICAR increase
phenformin increase

S37-p - H2B (mouse)
Orthologous residues
H2B (human): S37‑p, H2B (mouse): S37‑p, H2B (rat): S37‑p, H2B (cow): S36‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE AMPKA1 (mouse) genetic knockout/knockin of upstream enzyme, pharmacological activator of upstream enzyme, pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
2-deoxyglucose increase
AICAR increase
Downstream Regulation
 Effect of modification (process):  transcription, induced
 Comments:  S37 phosphorylation essential for cell survival in response to metabolic stress.


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