Curated Information

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Curated Information Page

PubMed Id: 20647423

This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus^®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus^®, click here.

Bungard D, et al. (2010) Signaling kinase AMPK activates stress-promoted transcription via histone H2B phosphorylation. Science 329, 1201-5 20647423

Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.

S37-p - H2B (human)

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Orthologous residues

H2B (human): S37‑p, H2B (mouse): S37‑p, H2B (rat): S37‑p, H2B (cow): S36‑p

Characterization

Methods used to characterize site in vivo: mutation of modification site, phospho-antibody, western blotting

Relevant cell lines - cell types - tissues: MEF (fibroblast)

Cellular systems studied: cell lines

Species studied: mouse

Enzymes shown to modify site in vitro:

Type	Enzyme
KINASE	AMPKA1 (mouse)

S79-p - ACC1 (mouse)

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Orthologous residues

ACC1 (human): S80‑p, ACC1 iso4 (human): S117‑p, ACC1 (mouse): S79‑p, ACC1 (rat): S79‑p, ACC1 iso2 (rat): S79‑p

Characterization

Methods used to characterize site in vivo: phospho-antibody, western blotting

Relevant cell lines - cell types - tissues: MEF (fibroblast)

Upstream Regulation

Treatments, proteins and their effect on site modification:

Treatments	Referenced Treatments	Manipulated Protein	Referenced Protein	Effect	Notes
UV				increase
ionizing radiation				increase
camptothecin				increase
adriamycin				increase
MNNG				increase
H2O2				increase
2-deoxyglucose				increase
AICAR				increase
phenformin				increase
2-deoxyglucose				increase
AICAR				increase

T183-p - AMPKA1 (mouse)

▲

Orthologous residues

AMPKA1 (human): T183‑p, AMPKA1 (mouse): T183‑p, AMPKA1 (rat): T183‑p, AMPKA1 (fruit fly): T184‑p

Characterization

Methods used to characterize site in vivo: phospho-antibody, western blotting

Relevant cell lines - cell types - tissues: MEF (fibroblast)

Upstream Regulation

Treatments, proteins and their effect on site modification:

Treatments	Referenced Treatments	Manipulated Protein	Referenced Protein	Effect	Notes
UV				increase
ionizing radiation				increase
camptothecin				increase
adriamycin				increase
MNNG				increase
H2O2				increase
2-deoxyglucose				increase
AICAR				increase
phenformin				increase

S37-p - H2B (mouse)

▲

Orthologous residues

H2B (human): S37‑p, H2B (mouse): S37‑p, H2B (rat): S37‑p, H2B (cow): S36‑p

Characterization

Methods used to characterize site in vivo: phospho-antibody, western blotting

Relevant cell lines - cell types - tissues: MEF (fibroblast)

Upstream Regulation

Potential in vivo enzymes for site:

Type	Enzyme	Evidence	Notes
KINASE	AMPKA1 (mouse)	pharmacological activator of upstream enzyme, genetic knockout/knockin of upstream enzyme, pharmacological inhibitor of upstream enzyme

Treatments, proteins and their effect on site modification:

Treatments	Referenced Treatments	Manipulated Protein	Referenced Protein	Effect	Notes
2-deoxyglucose				increase
AICAR				increase

Downstream Regulation

Effect of modification (process): transcription, altered

Comments: S37 phosphorylation essential for cell survival in response to metabolic stress.

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