Curated Information
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Curated Information Page
PubMed Id: 20818375 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Liu H, et al. (2010) Phosphorylation of MLL by ATR is required for execution of mammalian S-phase checkpoint. Nature 467, 343-6 20818375
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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K5-m3 - H3 (human)
Orthologous residues
H3 (human): K5‑m3, H3 (mouse): K5‑m3, H3 iso2 (mouse): K5‑m3, H3 iso3 (mouse): K5‑m3, H3 (rat): K5‑m3, H3 iso3 (rat): K5‑m3, H3 (pig): K5‑m3, H3 (chicken): K5‑m3, H3 (cow): K5‑m3
Characterization
 Methods used to characterize site in vivo modification-specific antibody
 Relevant cell lines - cell types - tissues:  293T (epithelial), Jurkat (T lymphocyte)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
METHYLTRANSFERASE MLL (human) siRNA inhibition of enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
etoposide increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
CDC45L (human) Disrupts co-immunoprecipitation

S516-p - MLL (human)
Orthologous residues
MLL (human): S516‑p, MLL (mouse): S514‑p, MLL iso5 (mouse): S547‑p, MLL (rat): S254‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE ATR (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATR (human) modification site within consensus motif, genetic knockout/knockin of upstream enzyme, phospho-motif antibody, pharmacological activator of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hydroxyurea increase
Downstream Regulation
 Effect of modification (function):  intracellular localization, molecular association, regulation, protein stabilization
 Effect of modification (process):  cell cycle regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SKP2 (human) Disrupts co-immunoprecipitation
 Comments:  accumulates on chromatin


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