Curated Information

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Curated Information Page

PubMed Id: 20818375

This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus^®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus^®, click here.

Liu H, et al. (2010) Phosphorylation of MLL by ATR is required for execution of mammalian S-phase checkpoint. Nature 467, 343-6 20818375

Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.

K5-m3 - H3 (human)

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Orthologous residues

H3 (human): K5‑m3, H3 (mouse): K5‑m3, H3 iso2 (mouse): K5‑m3, H3 iso3 (mouse): K5‑m3, H3 (rat): K5‑m3, H3 iso3 (rat): K5‑m3, H3 (pig): K5‑m3, H3 (chicken): K5‑m3, H3 (cow): K5‑m3

Characterization

Methods used to characterize site in vivo: modification-specific antibody

Relevant cell lines - cell types - tissues: 293T (epithelial), Jurkat (T lymphocyte)

Cellular systems studied: cell lines

Species studied: human

Upstream Regulation

Potential in vivo enzymes for site:

Type	Enzyme	Evidence	Notes
METHYLTRANSFERASE	MLL (human)	siRNA inhibition of enzyme

Treatments, proteins and their effect on site modification:

Treatments	Referenced Treatments	Manipulated Protein	Referenced Protein	Effect	Notes
etoposide				increase

Downstream Regulation

Effect of modification (function): molecular association, regulation

Modification regulates interactions with:

Interacting molecule	Interacting domains	Effect	Consequences (function)	Consequences (process)	Detection assays
CDC45L (human)		Disrupts			co-immunoprecipitation

S516-p - MLL (human)

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Orthologous residues

MLL (human): S516‑p, MLL (mouse): S514‑p, MLL iso5 (mouse): S547‑p, MLL (rat): S254‑p

Characterization

Methods used to characterize site in vivo: immunoprecipitation, mutation of modification site, phospho-antibody, western blotting

Relevant cell lines - cell types - tissues: 293T (epithelial)

Cellular systems studied: cell lines

Species studied: human

Enzymes shown to modify site in vitro:

Type	Enzyme
KINASE	ATR (human)

Upstream Regulation

Potential in vivo enzymes for site:

Type	Enzyme	Evidence	Notes
KINASE	ATR (human)	modification site within consensus motif, phospho-motif antibody, genetic knockout/knockin of upstream enzyme, pharmacological activator of upstream enzyme

Treatments, proteins and their effect on site modification:

Treatments	Referenced Treatments	Manipulated Protein	Referenced Protein	Effect	Notes
hydroxyurea				increase

Downstream Regulation

Effect of modification (function): intracellular localization, molecular association, regulation, protein stabilization

Effect of modification (process): cell cycle regulation

Modification regulates interactions with:

Interacting molecule	Interacting domains	Effect	Consequences (function)	Consequences (process)	Detection assays
SKP2 (human)		Disrupts			co-immunoprecipitation

Comments: accumulates on chromatin

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