Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Curated Information Page
PubMed Id: 20810654 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Marais A, et al. (2010) Cell Cycle-dependent Regulation of the Forkhead Transcription Factor FOXK2 by CDK{middle dot}Cyclin Complexes. J Biol Chem 285, 35728-39 20810654
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

S373-p - FOXK2 (human) ▲
Orthologous residues
FOXK2 (human): S373‑p, FOXK2 (mouse): S364‑p, FOXK2 (rat): S364‑p
Characterization
 Methods used to characterize site in vivo:  electrophoretic mobility shift, mass spectrometry (in vitro), mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  HeLa (cervical), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE CDK2 (human)
KINASE CDK1 (human)
 Comments:  as a CDK1-CLNB complex; as a CDK2-CLNA complex
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CDK1 (human) siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme
 Comments:  as a CDK1-CLNB complex
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase
roscovitine nocodazole inhibit treatment-induced increase
nocodazole increase
siRNA nocodazole inhibit treatment-induced increase siRNA CDK1
siRNA nocodazole inhibit treatment-induced increase siRNA CLNB
purvalanol nocodazole inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  protein stabilization
 Effect of modification (process):  apoptosis, altered, transcription, altered

T389-p - FOXK2 (human) ▲
Orthologous residues
FOXK2 (human): T389‑p, FOXK2 (mouse): T380‑p, FOXK2 (rat): T380‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, western blotting
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  HeLa (cervical), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human

S398-p - FOXK2 (human) ▲
Orthologous residues
FOXK2 (human): S398‑p, FOXK2 (mouse): S389‑p, FOXK2 (rat): S389‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, western blotting
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  HeLa (cervical), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human

S428-p - FOXK2 (human) ▲
Orthologous residues
FOXK2 (human): S428‑p, FOXK2 (mouse): S419‑p, FOXK2 (rat): S419‑p
Characterization
 Methods used to characterize site in vivo:  electrophoretic mobility shift, mass spectrometry (in vitro), mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE CDK2 (human)
KINASE CDK1 (human)
 Comments:  as a CDK1-CLNB complex; as a CDK2-CLNA complex
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase
roscovitine nocodazole inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  protein stabilization
 Effect of modification (process):  apoptosis, altered, transcription, altered


Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.