Curated Information
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Curated Information Page
PubMed Id: 20861316 
Dudek SM, et al. (2010) Abl Tyrosine Kinase Phosphorylates Nonmuscle Myosin Light Chain Kinase to Regulate Endothelial Barrier Function. Mol Biol Cell 21, 4042-4056 20861316
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

Y486-p - Cortactin (human)
Orthologous residues
Cortactin (human): Y486‑p, Cortactin iso3 (human): Y449‑p, Cortactin (mouse): Y482‑p, Cortactin (rat): Y444‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  EC (endothelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Abl (human) co-immunoprecipitation, siRNA inhibition of enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sphingosine 1-phosphate increase
siRNA decrease siRNA c-Abl

Y231-p - smMLCK (human)
Orthologous residues
smMLCK (human): Y231‑p, smMLCK iso2 (human): Y231‑p, smMLCK (mouse): Y226‑p, smMLCK (rat): Y235‑p, smMLCK (rabbit): , smMLCK (chicken): Y226‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry (in vitro)
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Abl (human)

Y464-p - smMLCK (human)
Orthologous residues
smMLCK (human): Y464‑p, smMLCK iso2 (human): , smMLCK (mouse): Y452‑p, smMLCK (rat): Y460‑p, smMLCK (rabbit): , smMLCK (chicken): Y478‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry (in vitro), phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  EC (endothelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Abl (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Abl (human) co-immunoprecipitation, siRNA inhibition of enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sphingosine 1-phosphate increase
siRNA decrease siRNA c-Abl

Y556-p - smMLCK (human)
Orthologous residues
smMLCK (human): Y556‑p, smMLCK iso2 (human): Y487‑p, smMLCK (mouse): F544‑p, smMLCK (rat): F552‑p, smMLCK (rabbit): , smMLCK (chicken): Y570‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry (in vitro)
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Abl (human)

Y611-p - smMLCK (human)
Orthologous residues
smMLCK (human): Y611‑p, smMLCK iso2 (human): Y542‑p, smMLCK (mouse): H599‑p, smMLCK (rat): H607‑p, smMLCK (rabbit): , smMLCK (chicken): G625‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry (in vitro)
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Abl (human)

Y792-p - smMLCK (human)
Orthologous residues
smMLCK (human): Y792‑p, smMLCK iso2 (human): Y723‑p, smMLCK (mouse): Y780‑p, smMLCK (rat): Y788‑p, smMLCK (rabbit): , smMLCK (chicken): Y805‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry (in vitro)
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Abl (human)

Y846-p - smMLCK (human)
Orthologous residues
smMLCK (human): Y846‑p, smMLCK iso2 (human): Y777‑p, smMLCK (mouse): H834‑p, smMLCK (rat): D842‑p, smMLCK (rabbit): , smMLCK (chicken): F858‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry (in vitro)
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Abl (human)

Y1449-p - smMLCK (human)
Orthologous residues
smMLCK (human): Y1449‑p, smMLCK iso2 (human): Y1380‑p, smMLCK (mouse): Y1471‑p, smMLCK (rat): Y1480‑p, smMLCK (rabbit): Y681‑p, smMLCK (chicken): Y1438‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry (in vitro)
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Abl (human)

Y1575-p - smMLCK (human)
Orthologous residues
smMLCK (human): Y1575‑p, smMLCK iso2 (human): Y1506‑p, smMLCK (mouse): Y1597‑p, smMLCK (rat): Y1606‑p, smMLCK (rabbit): Y807‑p, smMLCK (chicken): Y1564‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry (in vitro)
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Abl (human)

Y1635-p - smMLCK (human)
Orthologous residues
smMLCK (human): Y1635‑p, smMLCK iso2 (human): Y1566‑p, smMLCK (mouse): Y1657‑p, smMLCK (rat): Y1666‑p, smMLCK (rabbit): Y867‑p, smMLCK (chicken): Y1624‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry (in vitro)
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Abl (human)


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