Curated Information
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PubMed Id: 20516073 
Wang P, Zhu F, Lee NH, Konstantopoulos K (2010) Shear-induced interleukin-6 synthesis in chondrocytes: roles of E prostanoid (EP) 2 and EP3 in cAMP/protein kinase A- and PI3-K/Akt-dependent NF-kappaB activation. J Biol Chem 285, 24793-804 20516073
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

S473-p - Akt1 (human)
Orthologous residues
Akt1 (human): S473‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  T/C-28a2 (chondrocyte)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fluid shear stress PTGER2 (mouse) increase PTGER2 shRNA inhibits increase
fluid shear stress PTGER3 (mouse) PTGER2 (mouse) inhibit treatment-induced increase
AH6809 fluid shear stress PTGER2 (mouse) inhibit treatment-induced increase
sulprostone fluid shear stress PTGER2 (mouse) inhibit treatment-induced increase
fluid shear stress increase
wortmannin fluid shear stress inhibit treatment-induced increase
LY294002 fluid shear stress inhibit treatment-induced increase
H-89 fluid shear stress augment treatment-induced increase
NS398 fluid shear stress inhibit treatment-induced increase

S133-p - CREB (human)
Orthologous residues
CREB (human): S133‑p, CREB iso2 (human): S119‑p, CREB (mouse): S133‑p, CREB (rat): S133‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  T/C-28a2 (chondrocyte)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fluid shear stress increase
NS398 fluid shear stress no effect upon treatment-induced increase
wortmannin fluid shear stress no effect upon treatment-induced increase
LY294002 fluid shear stress no effect upon treatment-induced increase
H-89 fluid shear stress inhibit treatment-induced increase
fluid shear stress PTGER2 (mouse) increase PTGER2 shRNA inhibits increase
fluid shear stress PTGER3 (mouse) PTGER2 (mouse) inhibit treatment-induced increase
AH6809 fluid shear stress PTGER2 (mouse) inhibit treatment-induced increase
sulprostone fluid shear stress PTGER2 (mouse) inhibit treatment-induced increase

S276-p - NFkB-p65 (human)
Orthologous residues
NFkB‑p65 (human): S276‑p, NFkB‑p65 (mouse): S276‑p, NFkB‑p65 (rat): S276‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  T/C-28a2 (chondrocyte)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fluid shear stress PTGER2 (mouse) increase PTGER2 shRNA inhibits increase
fluid shear stress PTGER3 (mouse) PTGER2 (mouse) inhibit treatment-induced increase
AH6809 fluid shear stress PTGER2 (mouse) inhibit treatment-induced increase
sulprostone fluid shear stress PTGER2 (mouse) inhibit treatment-induced increase
fluid shear stress increase
wortmannin fluid shear stress no effect upon treatment-induced increase
LY294002 fluid shear stress augment treatment-induced increase
H-89 fluid shear stress inhibit treatment-induced increase
NS398 fluid shear stress inhibit treatment-induced increase

S536-p - NFkB-p65 (human)
Orthologous residues
NFkB‑p65 (human): S536‑p, NFkB‑p65 (mouse): S534‑p, NFkB‑p65 (rat): S535‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  T/C-28a2 (chondrocyte)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fluid shear stress PTGER2 (mouse) increase PTGER2 shRNA inhibits increase
fluid shear stress PTGER3 (mouse) PTGER2 (mouse) inhibit treatment-induced increase
AH6809 fluid shear stress PTGER2 (mouse) inhibit treatment-induced increase
sulprostone fluid shear stress PTGER2 (mouse) inhibit treatment-induced increase slight inhibition
wortmannin fluid shear stress PTGER2 (mouse) inhibit treatment-induced increase
LY294002 fluid shear stress PTGER2 (mouse) inhibit treatment-induced increase
H-89 fluid shear stress PTGER2 (mouse) augment treatment-induced increase
NS398 fluid shear stress PTGER2 (mouse) inhibit treatment-induced increase


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