Curated Information
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PubMed Id: 20606012 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Zheng Y, et al. (2010) Protein tyrosine kinase 6 directly phosphorylates AKT and promotes AKT activation in response to epidermal growth factor. Mol Cell Biol 30, 4280-92 20606012
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T308-p - Akt1 (human) ▲
Orthologous residues
Akt1 (human): T308‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Disease tissue studied:  prostate cancer
 Relevant cell lines - cell types - tissues:  293 (epithelial), BPH-1 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF Brk (human) increase

Y315-p - Akt1 (human) ▲
Orthologous residues
Akt1 (human): Y315‑p, Akt1 (mouse): Y315‑p, Akt1 (rat): Y315‑p, Akt1 (fruit fly): Y430‑p, Akt1 (cow): Y315‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry (in vitro), mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Brk (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Brk (human) co-immunoprecipitation, mutation in upstream enzyme recognition motif, transfection of constitutively active enzyme
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced
 Comments:  Brk induces Akt activation by phosphorylation on Y315,Y326.

Y326-p - Akt1 (human) ▲
Orthologous residues
Akt1 (human): Y326‑p, Akt1 (mouse): Y326‑p, Akt1 (rat): Y326‑p, Akt1 (fruit fly): Y441‑p, Akt1 (cow): Y326‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry (in vitro), mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Brk (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Brk (human) co-immunoprecipitation, mutation in upstream enzyme recognition motif, transfection of constitutively active enzyme
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced
 Comments:  Brk induces Akt activation by phosphorylation on Y315,Y326.

S473-p - Akt1 (human) ▲
Orthologous residues
Akt1 (human): S473‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Disease tissue studied:  prostate cancer
 Relevant cell lines - cell types - tissues:  293 (epithelial), BPH-1 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF Brk (human) increase

T308-p - Akt1 (mouse) ▲
Orthologous residues
Akt1 (human): T308‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  SYF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF Brk (human) increase

Y315-p - Akt1 (mouse) ▲
Orthologous residues
Akt1 (human): Y315‑p, Akt1 (mouse): Y315‑p, Akt1 (rat): Y315‑p, Akt1 (fruit fly): Y430‑p, Akt1 (cow): Y315‑p
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced
 Comments:  Brk induces Akt activation by phosphorylation on Y315,Y326.

Y326-p - Akt1 (mouse) ▲
Orthologous residues
Akt1 (human): Y326‑p, Akt1 (mouse): Y326‑p, Akt1 (rat): Y326‑p, Akt1 (fruit fly): Y441‑p, Akt1 (cow): Y326‑p
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced
 Comments:  Brk induces Akt activation by phosphorylation on Y315,Y326.

S473-p - Akt1 (mouse) ▲
Orthologous residues
Akt1 (human): S473‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  SYF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF Brk (human) increase

T203-p - ERK1 (mouse) ▲
Orthologous residues
ERK1 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  SYF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse

Y205-p - ERK1 (mouse) ▲
Orthologous residues
ERK1 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  SYF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse

T183-p - ERK2 (mouse) ▲
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  SYF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase
EGF Brk (mouse) no effect upon treatment-induced increase

Y185-p - ERK2 (mouse) ▲
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  SYF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase
EGF Brk (mouse) no effect upon treatment-induced increase


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