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Orthologous residues
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Chk1 (human): S296‑p, Chk1 (mouse): S296‑p, Chk1 (rat): S296‑p, Chk1 (chicken): S296‑p
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Characterization
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Methods used to characterize site in vivo:
immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
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|
Relevant cell lines - cell types - tissues:
HeLa (cervical)
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|
Cellular systems studied:
cell lines
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|
Species studied:
human
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Enzymes shown to modify site in vitro:
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|
|
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Comments:
autophosphorylation
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Upstream Regulation
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|
Treatments, proteins and their effect on site modification:
|
|
Treatments
|
Referenced Treatments
|
Manipulated Protein
|
Referenced Protein
|
Effect
|
Notes
|
|
UV
|
|
|
|
increase
|
|
|
ionizing radiation
|
|
|
|
increase
|
|
|
hydroxyurea
|
|
|
|
increase
|
|
|
aphidicolin
|
|
|
|
increase
|
|
|
actinomycin D
|
|
|
|
increase
|
|
|
mitomycin C
|
|
|
|
increase
|
slight increase
|
|
camptothecin
|
|
|
|
increase
|
|
|
doxorubicin
|
|
|
|
no change compared to control
|
|
|
|
Downstream Regulation
|
|
Effect of modification (function):
intracellular localization, molecular association, regulation
|
|
Effect of modification (process):
cell cycle regulation
|
|
Modification regulates interactions with:
|
|
Interacting molecule
|
Interacting domains
|
Effect
|
Consequences (function)
|
Consequences (process)
|
Detection assays
|
|
14-3-3 gamma (human)
|
|
Induces
|
molecular association, regulation
|
|
co-immunoprecipitation, in vitro
|
|
Cdc25A (human)
|
|
Induces
|
protein degradation, phosphorylation
|
|
co-immunoprecipitation
|
|
|
Comments:
Phosphorylated S296 is distributed thoroughout the nucleoplasm and is essential for DNA damage checkpoint.
|
