Curated Information
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Curated Information Page
PubMed Id: 20639859 
Kasahara K, et al. (2010) 14-3-3gamma mediates Cdc25A proteolysis to block premature mitotic entry after DNA damage. EMBO J 29, 2802-12 20639859
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S76-p - CDC25A (human)
Orthologous residues
CDC25A (human): S76‑p, CDC25A iso2 (human): S76‑p, CDC25A (mouse): S75‑p, CDC25A (rat): S76‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Chk1 (human)

T507-p - CDC25A (human)
Orthologous residues
CDC25A (human): T507‑p, CDC25A iso2 (human): T467‑p, CDC25A (mouse): T497‑p, CDC25A (rat): T508‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Chk1 (human)

S216-p - CDC25C (human)
Orthologous residues
CDC25C (human): S216‑p, CDC25C iso3 (human): S173‑p, CDC25C (mouse): , CDC25C (frog): S287‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV no change compared to control
MG132, UV no change compared to control

S296-p - Chk1 (human)
Orthologous residues
Chk1 (human): S296‑p, Chk1 (mouse): S296‑p, Chk1 (rat): S296‑p, Chk1 (chicken): S296‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Chk1 (human)
 Comments:  autophosphorylation
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
ionizing radiation increase
hydroxyurea increase
aphidicolin increase
actinomycin D increase
mitomycin C increase slight increase
camptothecin increase
doxorubicin no change compared to control
Downstream Regulation
 Effect of modification (function):  intracellular localization, molecular association, regulation
 Effect of modification (process):  cell cycle regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 gamma (human) Induces molecular association, regulation in vitro, co-immunoprecipitation
CDC25A (human) Induces protein degradation, phosphorylation co-immunoprecipitation
 Comments:  Phosphorylated S296 is distributed thoroughout the nucleoplasm and is essential for DNA damage checkpoint.

S317-p - Chk1 (human)
Orthologous residues
Chk1 (human): S317‑p, Chk1 (mouse): S317‑p, Chk1 (rat): S317‑p, Chk1 (chicken): S317‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
ionizing radiation increase
hydroxyurea increase
aphidicolin increase
actinomycin D increase
mitomycin C increase slight increase
camptothecin increase
doxorubicin no change compared to control

S345-p - Chk1 (human)
Orthologous residues
Chk1 (human): S345‑p, Chk1 (mouse): S345‑p, Chk1 (rat): S345‑p, Chk1 (chicken): S345‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
ionizing radiation increase
hydroxyurea increase
aphidicolin increase
actinomycin D increase
mitomycin C increase slight increase
camptothecin increase
doxorubicin no change compared to control


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