Curated Information
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Curated Information Page
PubMed Id: 20682768 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Nam SY, et al. (2010) Phosphorylation of CLK2 at serine 34 and threonine 127 by AKT controls cell survival after ionizing radiation. J Biol Chem 285, 31157-63 20682768
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S34-p - CLK2 (human)
Orthologous residues
CLK2 (human): S34‑p, CLK2 iso2 (human): S34‑p, CLK2 iso3 (human): S34‑p, CLK2 (mouse): S34‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  CCD-18Lu, HeLa (cervical)
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (human) co-immunoprecipitation, transfection of wild-type enzyme, modification site within consensus motif
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
ionizing radiation increase
Downstream Regulation
 Effect of modification (process):  apoptosis, altered, cell growth, altered

T127-p - CLK2 (human)
Orthologous residues
CLK2 (human): T127‑p, CLK2 iso2 (human): T127‑p, CLK2 iso3 (human): T127‑p, CLK2 (mouse): T127‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  CCD-18Lu, HeLa (cervical)
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (human) co-immunoprecipitation, transfection of wild-type enzyme, modification site within consensus motif
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
ionizing radiation increase
Downstream Regulation
 Effect of modification (process):  apoptosis, altered, cell growth, altered


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