Curated Information
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Curated Information Page
PubMed Id: 20513425 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Choo AY, et al. (2010) Glucose addiction of TSC null cells is caused by failed mTORC1-dependent balancing of metabolic demand with supply. Mol Cell 38, 487-99 20513425
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S79-p - ACC1 (mouse)
Orthologous residues
ACC1 (human): S80‑p, ACC1 iso4 (human): S117‑p, ACC1 (mouse): S79‑p, ACC1 iso2 (mouse): S117‑p, ACC1 (rat): S79‑p, ACC1 iso2 (rat): S79‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast) [TSC2 (mouse), homozygous knockout]
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Comments:  TSC2(-/-)/p53(-/-)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
rapamycin decrease TSC2 +/+MEFs

T183-p - AMPKA1 (mouse)
Orthologous residues
AMPKA1 (human): T183‑p, AMPKA1 (mouse): T183‑p, AMPKA1 (rat): T183‑p, AMPKA1 (fruit fly): T184‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast) [TSC2 (mouse), homozygous knockout]
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Comments:  TSC2(-/-)/p53(-/-)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
rapamycin decrease TSC2 +/+MEFs

T412-p - p70S6K (mouse)
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast) [TSC2 (mouse), homozygous knockout]
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Comments:  TSC2(-/-)/p53(-/-)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Bcl-xL (human) no change compared to control TSC2 -/- p53 -/- MEFs
rapamycin decrease
amino acid starvation decrease
L-glutamine withdrawal decrease
ouabain decrease slight decrease
cycloheximide no change compared to control
Raptor (human) increase siRNA decreases

S235-p - S6 (mouse)
Orthologous residues
S6 (human): S235‑p, S6 (mouse): S235‑p, S6 (rat): S235‑p, S6 (fruit fly): A234‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast) [TSC2 (mouse), homozygous knockout]
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Comments:  TSC2(-/-)/p53(-/-)

S236-p - S6 (mouse)
Orthologous residues
S6 (human): S236‑p, S6 (mouse): S236‑p, S6 (rat): S236‑p, S6 (fruit fly): S235‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast) [TSC2 (mouse), homozygous knockout]
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Comments:  TSC2(-/-)/p53(-/-)


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