Curated Information
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Curated Information Page
PubMed Id: 20664522 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Li H, et al. (2010) Phosphorylation of CLIP-170 by Plk1 and CK2 promotes timely formation of kinetochore-microtubule attachments. EMBO J 29, 2953-65 20664522
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S195-p - restin (human) ▲
Orthologous residues
restin (human): S195‑p, restin (mouse): S194‑p, restin (rat): S194‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE PLK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PLK1 (human) pharmacological inhibitor of upstream enzyme, siRNA inhibition of enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
siRNA decrease siRNA Plk1
nocodazole increase
BI2536 decrease
Downstream Regulation
 Effect of modification (function):  intracellular localization, phosphorylation
 Comments:  enhances phosphorylation of S1353

S1364-p - restin (human) ▲
Orthologous residues
restin (human): S1364‑p, restin (mouse): S1317‑p, restin (rat): S1246‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE PLK1 (human)
KINASE CK2-A1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2-A1 (human) pharmacological inhibitor of upstream enzyme, siRNA inhibition of enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
siRNA decrease siRNA CK2
TBCA decrease

S1246-p - restin (rat) ▲
Orthologous residues
restin (human): S1364‑p, restin (mouse): S1317‑p, restin (rat): S1246‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  HeLa (cervical), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
dynactin 1 (human) Induces co-immunoprecipitation


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