Curated Information
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Curated Information Page
PubMed Id: 20663873 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Cen B, et al. (2010) Regulation of Skp2 levels by the Pim-1 protein kinase. J Biol Chem 285, 29128-37 20663873
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S64-p - SKP2 (human)
Orthologous residues
SKP2 (human): S64‑p, SKP2 (mouse): S64‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Pim1 (rat)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Pim1 (human) transfection of wild-type enzyme, co-immunoprecipitation, pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
roscovitine decrease
wortmannin decrease
SMI-4a decrease
Downstream Regulation
 Effect of modification (function):  activity, induced, phosphorylation, protein stabilization, ubiquitination
 Effect of modification (process):  cell cycle regulation
 Comments:  p27 degradation; required for T417 phosphorylation

S72-p - SKP2 (human)
Orthologous residues
SKP2 (human): S72‑p, SKP2 (mouse): G72‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Pim1 (rat)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Pim1 (human) transfection of wild-type enzyme, co-immunoprecipitation, pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
roscovitine decrease
wortmannin decrease
SMI-4a decrease
Downstream Regulation
 Effect of modification (function):  phosphorylation, protein stabilization
 Effect of modification (process):  cell cycle regulation
 Comments:  required for T417 phosphorylation

T417-p - SKP2 (human)
Orthologous residues
SKP2 (human): T417‑p, SKP2 (mouse): T417‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Pim1 (rat)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Pim1 (human) transfection of wild-type enzyme, co-immunoprecipitation, pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
roscovitine decrease
wortmannin decrease
SMI-4a decrease
Downstream Regulation
 Effect of modification (function):  activity, induced, protein stabilization, ubiquitination
 Effect of modification (process):  cell cycle regulation
 Comments:  p27 degradation


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