Curated Information
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Curated Information Page
PubMed Id: 20659021 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Dzamko N, et al. (2010) Inhibition of LRRK2 kinase activity leads to dephosphorylation of Ser(910)/Ser(935), disruption of 14-3-3 binding and altered cytoplasmic localization. Biochem J 430, 405-13 20659021
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S910-p - LRRK2 (human) ▲
Orthologous residues
LRRK2 (human): S910‑p, LRRK2 iso3 (human): ‑, LRRK2 (mouse): S910‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Disease tissue studied:  Alzheimer's disease
 Relevant cell lines - cell types - tissues:  293 (epithelial), lymphoblastoid
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
H1152 decrease
sunitinib decrease
Downstream Regulation
 Effect of modification (function):  intracellular localization

S935-p - LRRK2 (human) ▲
Orthologous residues
LRRK2 (human): S935‑p, LRRK2 iso3 (human): ‑, LRRK2 (mouse): S935‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Disease tissue studied:  Alzheimer's disease
 Relevant cell lines - cell types - tissues:  293 (epithelial), lymphoblastoid
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
H1152 decrease
sunitinib decrease
Downstream Regulation
 Effect of modification (function):  intracellular localization

S910-p - LRRK2 (mouse) ▲
Orthologous residues
LRRK2 (human): S910‑p, LRRK2 iso3 (human): ‑, LRRK2 (mouse): S910‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
H1152 decrease
sunitinib decrease
GSK429286A no change compared to control
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 beta (human) Induces co-immunoprecipitation

S935-p - LRRK2 (mouse) ▲
Orthologous residues
LRRK2 (human): S935‑p, LRRK2 iso3 (human): ‑, LRRK2 (mouse): S935‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
H1152 decrease
sunitinib decrease
GSK429286A no change compared to control
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 beta (human) Induces co-immunoprecipitation


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