Curated Information
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Curated Information Page
PubMed Id: 20642453 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Nichols RJ, et al. (2010) 14-3-3 binding to LRRK2 is disrupted by multiple Parkinson's disease-associated mutations and regulates cytoplasmic localization. Biochem J 430, 393-404 20642453
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S860-p - LRRK2 (human)
Orthologous residues
LRRK2 (human): S860‑p, LRRK2 (mouse): S860‑p, LRRK2 (rat): S859‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S910-p - LRRK2 (human)
Orthologous residues
LRRK2 (human): S910‑p, LRRK2 (mouse): S910‑p, LRRK2 (rat): S909‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  intracellular localization, molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 theta (human) Induces co-immunoprecipitation
14-3-3 epsilon (human) Induces co-immunoprecipitation
14-3-3 eta (human) Induces co-immunoprecipitation
14-3-3 zeta (human) Induces co-immunoprecipitation
14-3-3 beta (human) Induces co-immunoprecipitation

S935-p - LRRK2 (human)
Orthologous residues
LRRK2 (human): S935‑p, LRRK2 (mouse): S935‑p, LRRK2 (rat): S934‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  intracellular localization, molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 epsilon (human) Induces co-immunoprecipitation
14-3-3 zeta (human) Induces co-immunoprecipitation
14-3-3 beta (human) Induces co-immunoprecipitation
14-3-3 theta (human) Induces co-immunoprecipitation
14-3-3 eta (human) Induces co-immunoprecipitation

S955-p - LRRK2 (human)
Orthologous residues
LRRK2 (human): S955‑p, LRRK2 (mouse): S955‑p, LRRK2 (rat): S954‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S973-p - LRRK2 (human)
Orthologous residues
LRRK2 (human): S973‑p, LRRK2 (mouse): S973‑p, LRRK2 (rat): S972‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S976-p - LRRK2 (human)
Orthologous residues
LRRK2 (human): S976‑p, LRRK2 (mouse): S976‑p, LRRK2 (rat): S975‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S860-p - LRRK2 (mouse)
Orthologous residues
LRRK2 (human): S860‑p, LRRK2 (mouse): S860‑p, LRRK2 (rat): S859‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse

S910-p - LRRK2 (mouse)
Orthologous residues
LRRK2 (human): S910‑p, LRRK2 (mouse): S910‑p, LRRK2 (rat): S909‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast), brain, liver, spleen
 Cellular systems studied:  cell lines, tissue
 Species studied:  mouse
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 eta (mouse) Induces co-immunoprecipitation
14-3-3 epsilon (mouse) Induces co-immunoprecipitation
14-3-3 zeta (mouse) Induces co-immunoprecipitation
14-3-3 beta (mouse) Induces co-immunoprecipitation
14-3-3 theta (mouse) Induces co-immunoprecipitation
Associated Diseases
Diseases: Alterations: Comments:
Parkinson's disease decreased phosphorylation of this site and interaction with 14-3-3 were abolished in the LRRK2[R1441C] knockin mice

S935-p - LRRK2 (mouse)
Orthologous residues
LRRK2 (human): S935‑p, LRRK2 (mouse): S935‑p, LRRK2 (rat): S934‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast), brain, liver, spleen
 Cellular systems studied:  cell lines, tissue
 Species studied:  mouse
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 eta (mouse) Induces co-immunoprecipitation
14-3-3 epsilon (mouse) Induces co-immunoprecipitation
14-3-3 zeta (mouse) Induces co-immunoprecipitation
14-3-3 beta (mouse) Induces co-immunoprecipitation
14-3-3 theta (mouse) Induces co-immunoprecipitation
Associated Diseases
Diseases: Alterations: Comments:
Parkinson's disease decreased phosphorylation of this site and interaction with 14-3-3 were abolished in the LRRK2[R1441C] knockin mice


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