Curated Information
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Curated Information Page
PubMed Id: 20622854 
Liu W, et al. (2010) PHF8 mediates histone H4 lysine 20 demethylation events involved in cell cycle progression. Nature 466, 508-12 20622854
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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K10-m1 - H3 (human)
Orthologous residues
H3 (human): K10‑m1, H3 (mouse): K10‑m1, H3 iso2 (mouse): K10‑m1, H3 iso3 (mouse): K10‑m1, H3 (rat): K10‑m1, H3 iso3 (rat): K10‑m1, H3 (pig): K10‑m1, H3 (chicken): K10‑m1, H3 (cow): K10‑m1
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  HeLa (cervical), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
DEMETHYLASE PHF8 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
DEMETHYLASE PHF8 (human) siRNA inhibition of enzyme
Downstream Regulation
 Effect of modification (process):  cell cycle regulation

K10-m2 - H3 (human)
Orthologous residues
H3 (human): K10‑m2, H3 (mouse): K10‑m2, H3 iso2 (mouse): K10‑m2, H3 iso3 (mouse): K10‑m2, H3 (rat): K10‑m2, H3 iso3 (rat): K10‑m2, H3 (pig): K10‑m2, H3 (chicken): K10‑m2, H3 (cow): K10‑m2
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  HeLa (cervical), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
DEMETHYLASE PHF8 (human)

K28-m2 - H3 (human)
Orthologous residues
H3 (human): K28‑m2, H3 (mouse): K28‑m2, H3 iso2 (mouse): K28‑m2, H3 iso3 (mouse): K28‑m2, H3 (rat): K28‑m2, H3 iso3 (rat): K28‑m2, H3 (pig): K28‑m2, H3 (chicken): K28‑m2, H3 (cow): K28‑m2
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  HeLa (cervical), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
DEMETHYLASE PHF8 (human)

K21-m1 - H4 (human)
Orthologous residues
H4 (human): K21‑m1, H4 (mouse): K21‑m1, H4 (rat): K21‑m1, H4 (pig): K21‑m1, H4 (cow): K21‑m1
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  HeLa (cervical), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
DEMETHYLASE PHF8 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
DEMETHYLASE PHF8 (human) siRNA inhibition of enzyme
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  cell cycle regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
NCAPD3 (human) Induces pull-down assay
LUZP5 (human) Induces pull-down assay

S69-p - PHF8 (human)
Orthologous residues
PHF8 (human): S69‑p, PHF8 (mouse): S33‑p, PHF8 (rat): S33‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry (in vitro), mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)

S120-p - PHF8 (human)
Orthologous residues
PHF8 (human): S120‑p, PHF8 (mouse): S84‑p, PHF8 (rat): S84‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry (in vitro), mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)


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