Curated Information
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Curated Information Page
PubMed Id: 20713600 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Cherra SJ, et al. (2010) Regulation of the autophagy protein LC3 by phosphorylation. J Cell Biol 190, 533-9 20713600
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S12-p - LC3A (human) ▲
Orthologous residues
LC3A (human): S12‑p, LC3A (mouse): S12‑p, LC3A (rat): S12‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  neuroblastoma
 Relevant cell lines - cell types - tissues:  HeLa (cervical), SH-SY5Y (neural crest)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKACa (human) pharmacological inhibitor of upstream enzyme, pharmacological activator of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
forskolin increase
H-89 forskolin inhibit treatment-induced increase
rapamycin decrease
MPP+ decrease
Downstream Regulation
 Effect of modification (function):  intracellular localization
 Effect of modification (process):  autophagy, altered

S12-p - LC3A (mouse) ▲
Orthologous residues
LC3A (human): S12‑p, LC3A (mouse): S12‑p, LC3A (rat): S12‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'neuron, cortical'
 Cellular systems studied:  primary cultured cells
 Species studied:  mouse
Downstream Regulation
 Effect of modification (function):  intracellular localization
 Effect of modification (process):  autophagy, altered

S12-p - LC3A (rat) ▲
Orthologous residues
LC3A (human): S12‑p, LC3A (mouse): S12‑p, LC3A (rat): S12‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry (in vitro)
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE PKACa (human)


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