Curated Information
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Curated Information Page
PubMed Id: 20513353 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Hill EV, et al. (2010) Regulation of PIKfyve phosphorylation by insulin and osmotic stress. Biochem Biophys Res Commun 397, 650-5 20513353
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S105-p - PIKFYVE iso1 (mouse)
Orthologous residues
PIKFYVE (human): S105‑p, PIKFYVE iso2 (human): , PIKFYVE (mouse): S105‑p, PIKFYVE iso1 (mouse): S105‑p, PIKFYVE iso3 (mouse): S105‑p, PIKFYVE (rat): S105‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry (in vitro), mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  CHO (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  hamster
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)

S318-p - PIKFYVE iso1 (mouse)
Orthologous residues
PIKFYVE (human): S307‑p, PIKFYVE iso2 (human): , PIKFYVE (mouse): S307‑p, PIKFYVE iso1 (mouse): S318‑p, PIKFYVE iso3 (mouse): S307‑p, PIKFYVE (rat): S318‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry (in vitro), mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  CHO (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  hamster
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
wortmannin no change compared to control
YM201636 no change compared to control
insulin increase
wortmannin insulin inhibit treatment-induced increase
YM201636 insulin inhibit treatment-induced increase
Akt-I-1,2 insulin inhibit treatment-induced increase
sorbitol increase
wortmannin sorbitol no effect upon treatment-induced increase
YM201636 sorbitol no effect upon treatment-induced increase
Akt-I-1,2 sorbitol no effect upon treatment-induced increase


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