Curated Information
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Curated Information Page
PubMed Id: 20585028 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Gallazzini M, et al. (2010) c-Abl mediates high NaCl-induced phosphorylation and activation of the transcription factor TonEBP/OREBP. FASEB J 24, 4325-35 20585028
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y226-p - Abl (human) ▲
Orthologous residues
Abl (human): Y226‑p, Abl iso2 (human): Y245‑p, Abl (mouse): Y226‑p, Abl iso2 (mouse): Y245‑p, Abl (rat): Y226‑p, Abl iso2 (rat): Y245‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hypertonic buffer increase

Y393-p - Abl (human) ▲
Orthologous residues
Abl (human): Y393‑p, Abl iso2 (human): Y412‑p, Abl (mouse): Y393‑p, Abl iso2 (mouse): Y412‑p, Abl (rat): Y393‑p, Abl iso2 (rat): Y412‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hypertonic buffer increase

Y143-p - NFAT5 (human) ▲
Orthologous residues
NFAT5 (human): Y143‑p, NFAT5 (mouse): Y143‑p, NFAT5 iso2 (mouse): Y161‑p, NFAT5 (rat): Y160‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Abl (human) siRNA inhibition of enzyme, modification site within consensus motif, pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hypertonic buffer increase
imatinib hypertonic buffer inhibit treatment-induced increase
siRNA hypertonic buffer inhibit treatment-induced increase c-Abl siRNA
Downstream Regulation
 Effect of modification (function):  intracellular localization

Y160-p - NFAT5 (rat) ▲
Orthologous residues
NFAT5 (human): Y143‑p, NFAT5 (mouse): Y143‑p, NFAT5 iso2 (mouse): Y161‑p, NFAT5 (rat): Y160‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'kidney, inner medulla', 293 (epithelial)
 Cellular systems studied:  cell lines, tissue
 Species studied:  human, rat
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Abl (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Abl (human) co-immunoprecipitation, transfection of wild-type enzyme, transfection of inactive enzyme, modification site within consensus motif
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
dDAVP increase
hypertonic buffer increase
Downstream Regulation
 Effect of modification (function):  activation, intracellular localization
 Effect of modification (process):  transcription, altered


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