|
Orthologous residues
|
|
HPK1 (human): Y381‑p, HPK1 (mouse): Y379‑p
|
|
Characterization
|
|
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, phospho-antibody
|
|
Relevant cell lines - cell types - tissues:
B lymphocyte-spleen, COS (fibroblast), WEHI-231 (B lymphocyte)
|
|
Cellular systems studied:
cell lines
|
|
Species studied:
human, monkey
|
|
Upstream Regulation
|
|
Potential in vivo enzymes for site:
|
|
Type
|
Enzyme
|
Evidence
|
Notes
|
|
KINASE
|
Syk (mouse)
|
genetic knockout/knockin of upstream enzyme, genetic transfer of wild-type enzyme
|
|
|
|
Treatments, proteins and their effect on site modification:
|
|
Treatments
|
Referenced Treatments
|
Manipulated Protein
|
Referenced Protein
|
Effect
|
Notes
|
|
anti-IgM
|
|
|
|
increase
|
|
|
|
Downstream Regulation
|
|
Effect of modification (function):
enzymatic activity, induced, molecular association, regulation
|
|
Modification regulates interactions with:
|
|
Interacting molecule
|
Interacting domains
|
Effect
|
Consequences (function)
|
Consequences (process)
|
Detection assays
|
|
BLNK (mouse)
|
|
Induces
|
activation
|
|
co-immunoprecipitation, electrophoretic visualization
|
|
