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PubMed Id: 20427654

This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus^®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus^®, click here.

Kurup P, et al. (2010) Abeta-mediated NMDA receptor endocytosis in Alzheimer's disease involves ubiquitination of the tyrosine phosphatase STEP61. J Neurosci 30, 5948-57 20427654

Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.

Y1474-p - NMDAR2B (human)

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Orthologous residues

NMDAR2B (human): Y1474‑p, NMDAR2B (mouse): Y1472‑p, NMDAR2B (rat): Y1472‑p

Characterization

Enzymes shown to modify site in vitro:

Type	Enzyme
PHOSPHATASE	STEP (human)

S245-p - STEP (human)

▲

Orthologous residues

STEP (human): S245‑p, STEP (mouse): S221‑p, STEP (rat): S49‑p

Characterization

Methods used to characterize site in vivo: mutation of modification site

Relevant cell lines - cell types - tissues: 293 (epithelial)

Cellular systems studied: cell lines

Species studied: human

Downstream Regulation

Effect of modification (function): enzymatic activity, inhibited

Y1472-p - NMDAR2B (mouse)

▲

Orthologous residues

NMDAR2B (human): Y1474‑p, NMDAR2B (mouse): Y1472‑p, NMDAR2B (rat): Y1472‑p

Characterization

Methods used to characterize site in vivo: phospho-antibody, western blotting

Relevant cell lines - cell types - tissues: 'brain, cortex'

Cellular systems studied: tissue

Species studied: mouse

Upstream Regulation

Potential in vivo enzymes for site:

Type	Enzyme	Evidence	Notes
PHOSPHATASE	STEP (mouse)	activation of upstream enzyme

Treatments, proteins and their effect on site modification:

Treatments	Referenced Treatments	Manipulated Protein	Referenced Protein	Effect	Notes
beta-amyloid				decrease

Downstream Regulation

Effect of modification (function): intracellular localization

Associated Diseases

Diseases:	Alterations:	Comments:
Alzheimer's disease	decreased	result of increased levels and activity of STEP phosphatase in aged Tg2576 mice

S49-p - STEP (rat)

▲

Orthologous residues

STEP (human): S245‑p, STEP (mouse): S221‑p, STEP (rat): S49‑p

Characterization

Methods used to characterize site in vivo: phospho-antibody, western blotting

Relevant cell lines - cell types - tissues: 'brain, cortex'

Cellular systems studied: tissue

Species studied: mouse

Upstream Regulation

Treatments, proteins and their effect on site modification:

Treatments	Referenced Treatments	Manipulated Protein	Referenced Protein	Effect	Notes
beta-amyloid				decrease

Downstream Regulation

Effect of modification (function): enzymatic activity, inhibited, ubiquitination

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