Curated Information
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PubMed Id: 20424160 
Hegazy SA, et al. (2010) The tyrosine 343 residue of nucleophosmin (NPM)-anaplastic lymphoma kinase (ALK) is important for its interaction with SHP1, a cytoplasmic tyrosine phosphatase with tumor suppressor functions. J Biol Chem 285, 19813-20 20424160
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
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Y338-p - NPM-ALK (human)
Orthologous residues
NPM‑ALK (human): Y338‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Disease tissue studied:  lymphoma, T cell lymphoma
 Relevant cell lines - cell types - tissues:  GP293 (fibroblast), Karpas-299 (T lymphocyte)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE SHP-1 (human) phospho-antibody, co-immunoprecipitation SHP-1 likely dephosphorylated Y342, Y338 and other tyrosines (not Y343). data suggests SHP-1 directly dephosphorylates ALK, but may be indirect.

Y342-p - NPM-ALK (human)
Orthologous residues
NPM‑ALK (human): Y342‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Disease tissue studied:  lymphoma, T cell lymphoma
 Relevant cell lines - cell types - tissues:  GP293 (fibroblast), Karpas-299 (T lymphocyte)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE SHP-1 (human) phospho-antibody, co-immunoprecipitation SHP-1 likely dephosphorylated Y342, Y338 and other tyrosines (not Y343). data suggests SHP-1 directly dephosphorylates ALK, but may be indirect.

Y343-p - NPM-ALK (human)
Orthologous residues
NPM‑ALK (human): Y343‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Disease tissue studied:  lymphoma, T cell lymphoma
 Relevant cell lines - cell types - tissues:  GP293 (fibroblast), Karpas-299 (T lymphocyte)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  carcinogenesis, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SHP-1 (human) SH2 Induces phosphorylation carcinogenesis, altered co-immunoprecipitation
 Comments:  SHP1 phosphatase mediates tyrosine dephosphorylation of ALK


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