Curated Information
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Curated Information Page
PubMed Id: 20424160 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Hegazy SA, et al. (2010) The tyrosine 343 residue of nucleophosmin (NPM)-anaplastic lymphoma kinase (ALK) is important for its interaction with SHP1, a cytoplasmic tyrosine phosphatase with tumor suppressor functions. J Biol Chem 285, 19813-20 20424160
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y338-p - NPM-ALK (human) ▲
Orthologous residues
NPM‑ALK (human): Y338‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting
 Disease tissue studied:  lymphoma, T cell lymphoma
 Relevant cell lines - cell types - tissues:  GP293 (fibroblast), Karpas-299 (T lymphocyte)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE SHP-1 (human) phospho-antibody, co-immunoprecipitation SHP-1 likely dephosphorylated Y342, Y338 and other tyrosines (not Y343). data suggests SHP-1 directly dephosphorylates ALK, but may be indirect.

Y342-p - NPM-ALK (human) ▲
Orthologous residues
NPM‑ALK (human): Y342‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting
 Disease tissue studied:  lymphoma, T cell lymphoma
 Relevant cell lines - cell types - tissues:  GP293 (fibroblast), Karpas-299 (T lymphocyte)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE SHP-1 (human) phospho-antibody, co-immunoprecipitation SHP-1 likely dephosphorylated Y342, Y338 and other tyrosines (not Y343). data suggests SHP-1 directly dephosphorylates ALK, but may be indirect.

Y343-p - NPM-ALK (human) ▲
Orthologous residues
NPM‑ALK (human): Y343‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting
 Disease tissue studied:  lymphoma, T cell lymphoma
 Relevant cell lines - cell types - tissues:  GP293 (fibroblast), Karpas-299 (T lymphocyte)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  carcinogenesis, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SHP-1 (human) SH2 Induces phosphorylation carcinogenesis, altered co-immunoprecipitation
 Comments:  SHP1 phosphatase mediates tyrosine dephosphorylation of ALK


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