Curated Information
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Curated Information Page
PubMed Id: 20345371 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Gehret AU, Hinkle PM (2010) Importance of regions outside the cytoplasmic tail of G-protein-coupled receptors for phosphorylation and dephosphorylation. Biochem J 428, 235-45 20345371
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S262-p - ADRB2 (human)
Orthologous residues
ADRB2 (human): S262‑p, ADRB2 (mouse): S262‑p, ADRB2 (rat): S262‑p, ADRB2 (hamster): S262‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  TRHR-ADRB2 chimearic protein
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
isoproterenol increase
H-89 isoproterenol inhibit treatment-induced increase

S355-p - ADRB2 (human)
Orthologous residues
ADRB2 (human): S355‑p, ADRB2 (mouse): S355‑p, ADRB2 (rat): S355‑p, ADRB2 (hamster): S355‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  TRHR-ADRB2 chimearic protein
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
isoproterenol increase
TRH increase
Downstream Regulation
 Effect of modification (function):  receptor desensitization, altered, receptor internalization, altered

S356-p - ADRB2 (human)
Orthologous residues
ADRB2 (human): S356‑p, ADRB2 (mouse): S356‑p, ADRB2 (rat): S356‑p, ADRB2 (hamster): S356‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  TRHR-ADRB2 chimearic protein
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
isoproterenol increase
TRH increase
Downstream Regulation
 Effect of modification (function):  receptor desensitization, altered, receptor internalization, altered

S355-p - TRHR (rat)
Orthologous residues
TRHR (human): S355‑p, TRHR (mouse): S355‑p, TRHR (rat): S355‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  ADRB2-TRHR chimearic protein
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TRH increase
Downstream Regulation
 Effect of modification (function):  receptor internalization, altered

S360-p - TRHR (rat)
Orthologous residues
TRHR (human): S360‑p, TRHR (mouse): S360‑p, TRHR (rat): S360‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  ADRB2-TRHR chimearic protein
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TRH increase
Downstream Regulation
 Effect of modification (function):  receptor internalization, altered

S364-p - TRHR (rat)
Orthologous residues
TRHR (human): S364‑p, TRHR (mouse): S364‑p, TRHR (rat): S364‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  ADRB2-TRHR chimearic protein
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TRH increase
Downstream Regulation
 Effect of modification (function):  receptor internalization, altered

T365-p - TRHR (rat)
Orthologous residues
TRHR (human): T365‑p, TRHR (mouse): T365‑p, TRHR (rat): T365‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  ADRB2-TRHR chimearic protein
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
isoproterenol increase
TRH increase
Downstream Regulation
 Effect of modification (function):  receptor internalization, altered


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