Curated Information
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Curated Information Page
PubMed Id: 20472934 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Dovas A, et al. (2010) Serine 34 phosphorylation of rho guanine dissociation inhibitor (RhoGDIalpha) links signaling from conventional protein kinase C to RhoGTPase in cell adhesion. J Biol Chem 285, 23296-308 20472934
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S34-p - RhoGDI alpha (human)
Orthologous residues
RhoGDI alpha (human): S34‑p, RhoGDI alpha iso3 (human): S34‑p, RhoGDI alpha (mouse): S34‑p, RhoGDI alpha (rat): S34‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  leukemia, chronic myelogenous leukemia
 Relevant cell lines - cell types - tissues:  K562 (erythroid), MDCK (epithelial), S2
 Cellular systems studied:  cell lines
 Species studied:  dog, fruit fly, human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCA (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol ester increase
Go 6976 phorbol ester inhibit treatment-induced increase
GF109203X phorbol ester inhibit treatment-induced increase
HGF increase
Go 6976 HGF inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  cell adhesion, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
RhoA (human) Disrupts pull-down assay

S34-p - RhoGDI alpha (rat)
Orthologous residues
RhoGDI alpha (human): S34‑p, RhoGDI alpha iso3 (human): S34‑p, RhoGDI alpha (mouse): S34‑p, RhoGDI alpha (rat): S34‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  fibroblast-embryo
 Cellular systems studied:  primary cultured cells
 Species studied:  rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
U73122 decrease


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