Curated Information
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Curated Information Page
PubMed Id: 20144759 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Nakamura T, et al. (2010) Double-stranded RNA-dependent protein kinase links pathogen sensing with stress and metabolic homeostasis. Cell 140, 338-48 20144759
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S473-p - Akt1 (mouse) ▲
Orthologous residues
Akt1 (human): S473‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Disease tissue studied:  type 2 diabetes
 Relevant cell lines - cell types - tissues:  MEF (fibroblast) [PKR (mouse), homozygous knockout]
 Cellular systems studied:  primary cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin PKR (mouse) decrease PKR -/- increases

S52-p - eIF2-alpha (mouse) ▲
Orthologous residues
eIF2‑alpha (human): S52‑p, eIF2‑alpha (mouse): S52‑p, eIF2‑alpha (rat): S52‑p, eIF2‑alpha (rabbit): S51‑p, eIF2‑alpha (fruit fly): S51‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Disease tissue studied:  type 2 diabetes
 Relevant cell lines - cell types - tissues:  MEF (fibroblast) [PKR (mouse), homozygous knockout]
 Cellular systems studied:  primary cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PKR (mouse) increase PKR -/- decreases

S307-p - IRS1 (mouse) ▲
Orthologous residues
IRS1 (human): S312‑p, IRS1 (mouse): S307‑p, IRS1 (rat): S307‑p
Characterization
 Methods used to characterize site in vivo:  [32P] ATP in vitro, phospho-antibody, western blotting
 Disease tissue studied:  type 2 diabetes
 Relevant cell lines - cell types - tissues:  MEF (fibroblast) [JNK1 (mouse), homozygous knockout], MEF (fibroblast) [PKR (mouse), homozygous knockout]
 Cellular systems studied:  primary cells
 Species studied:  mouse
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE PKR (mouse)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKR (human) phospho-antibody, co-immunoprecipitation, transfection of wild-type enzyme
KINASE PKR (mouse) activation of upstream enzyme, co-immunoprecipitation, genetic knockout/knockin of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
palmitate increase
thapsigargin increase
JNK1 (mouse) increase JNK -/- decreases
TNF decrease
Downstream Regulation
 Comments:  insulin resistance

T183-p - JNK1 (mouse) ▲
Orthologous residues
JNK1 (human): T183‑p, JNK1 iso2 (human): T183‑p, JNK1 iso3 (human): T183‑p, JNK1 (mouse): T183‑p, JNK1 (rat): T183‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Disease tissue studied:  type 2 diabetes
 Relevant cell lines - cell types - tissues:  MEF (fibroblast) [PKR (mouse), homozygous knockout]
 Cellular systems studied:  primary cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
thapsigargin increase
thapsigargin PKR (mouse) increase PKR -/- decrreases

Y185-p - JNK1 (mouse) ▲
Orthologous residues
JNK1 (human): Y185‑p, JNK1 iso2 (human): Y185‑p, JNK1 iso3 (human): Y185‑p, JNK1 (mouse): Y185‑p, JNK1 (rat): Y185‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Disease tissue studied:  type 2 diabetes
 Relevant cell lines - cell types - tissues:  MEF (fibroblast) [PKR (mouse), homozygous knockout]
 Cellular systems studied:  primary cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
thapsigargin increase
thapsigargin PKR (mouse) increase PKR -/- decrreases


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