Curated Information
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PubMed Id: 20227039 
Hsieh AC, et al. (2010) Genetic dissection of the oncogenic mTOR pathway reveals druggable addiction to translational control via 4EBP-eIF4E. Cancer Cell 17, 249-61 20227039
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T36-p - 4E-BP1 (mouse)
Orthologous residues
4E‑BP1 (human): T37‑p, 4E‑BP1 (mouse): T36‑p, 4E‑BP1 (rat): T36‑p, 4E‑BP1 (fruit fly): T37‑p
Characterization
 Methods used to characterize site in vivo peptide sequencing, western blotting
 Relevant cell lines - cell types - tissues:  thymocyte
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Comments:  thymocytes and tumors from subcutaneous injection of thymocytes (with activated Akt2)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Akt2 (mouse) increase
rapamycin decrease
PP242 decrease

T45-p - 4E-BP1 (mouse)
Orthologous residues
4E‑BP1 (human): T46‑p, 4E‑BP1 (mouse): T45‑p, 4E‑BP1 (rat): T45‑p, 4E‑BP1 (fruit fly): T46‑p
Characterization
 Methods used to characterize site in vivo peptide sequencing, western blotting
 Relevant cell lines - cell types - tissues:  thymocyte
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Comments:  thymocytes and tumors from subcutaneous injection of thymocytes (with activated Akt2)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Akt2 (mouse) increase
rapamycin decrease
PP242 decrease

S473-p - Akt1 (mouse)
Orthologous residues
Akt1 (human): S473‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
 Methods used to characterize site in vivo peptide sequencing, western blotting
 Relevant cell lines - cell types - tissues:  thymocyte
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Comments:  thymocytes and tumors from subcutaneous injection of thymocytes (with activated Akt2)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Akt2 (mouse) increase
rapamycin decrease slight decrease
PP242 decrease

S240-p - S6 (mouse)
Orthologous residues
S6 (human): S240‑p, S6 (mouse): S240‑p, S6 (rat): S240‑p, S6 (fruit fly): S239‑p
Characterization
 Methods used to characterize site in vivo peptide sequencing, western blotting
 Relevant cell lines - cell types - tissues:  thymocyte
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Comments:  thymocytes and tumors from subcutaneous injection of thymocytes (with activated Akt2)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
rapamycin decrease
PP242 decrease

S244-p - S6 (mouse)
Orthologous residues
S6 (human): S244‑p, S6 (mouse): S244‑p, S6 (rat): S244‑p, S6 (fruit fly): V243‑p
Characterization
 Methods used to characterize site in vivo peptide sequencing, western blotting
 Relevant cell lines - cell types - tissues:  thymocyte
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Comments:  thymocytes and tumors from subcutaneous injection of thymocytes (with activated Akt2)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
rapamycin decrease
PP242 decrease


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