Curated Information
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PubMed Id: 20348946 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Honaker Y, Piwnica-Worms H (2010) Casein kinase 1 functions as both penultimate and ultimate kinase in regulating Cdc25A destruction. Oncogene 29, 3324-34 20348946
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S76-p - Cdc25A (human)
Orthologous residues
Cdc25A (human): S76‑p, Cdc25A iso2 (human): S76‑p, Cdc25A (mouse): S75‑p, Cdc25A (rat): S76‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HeLa (cervical) [Cdc25A (human), transfection]
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, phosphorylation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
BTRC (human) Induces co-immunoprecipitation
 Comments:  S76, S79, and S82 phosphorylation affect binding to BTRC; S79 phosphorylation by CK1-A primes for S82 phosphorylation by CK1-A; S79 phosphorylation is dependent on S76.

S79-p - Cdc25A (human)
Orthologous residues
Cdc25A (human): S79‑p, Cdc25A iso2 (human): S79‑p, Cdc25A (mouse): S78‑p, Cdc25A (rat): S79‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HeLa (cervical) [Cdc25A (human), transfection]
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK1A (human)
 Comments:  S79 phosphorylation primes for S82 phosphorylation.
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK1A (human) phospho-antibody, transfection of wild-type enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
D4476 decrease
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, phosphorylation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
BTRC (human) Induces co-immunoprecipitation
 Comments:  S76, S79, and S82 phosphorylation affect binding to BTRC; S79 phosphorylation by CK1-A primes for S82 phosphorylation by CK1-A; S79 phosphorylation is dependent on S76.

T80-p - Cdc25A (human)
Orthologous residues
Cdc25A (human): T80‑p, Cdc25A iso2 (human): T80‑p, Cdc25A (mouse): T79‑p, Cdc25A (rat): T80‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HeLa (cervical) [Cdc25A (human), transfection]
 Cellular systems studied:  cell lines
 Species studied:  human

S82-p - Cdc25A (human)
Orthologous residues
Cdc25A (human): S82‑p, Cdc25A iso2 (human): S82‑p, Cdc25A (mouse): S81‑p, Cdc25A (rat): S82‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HeLa (cervical) [Cdc25A (human), transfection]
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK1A (human)
 Comments:  S79 phosphorylation primes for S82 phosphorylation.
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK1A (human) phospho-antibody, pharmacological inhibitor of upstream enzyme, transfection of wild-type enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
D4476 decrease
ionizing radiation increase
D4476 ionizing radiation inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
BTRC (human) Induces co-immunoprecipitation
 Comments:  S76, S79, and S82 phosphorylation affect binding to BTRC

S88-p - Cdc25A (human)
Orthologous residues
Cdc25A (human): S88‑p, Cdc25A iso2 (human): S88‑p, Cdc25A (mouse): S87‑p, Cdc25A (rat): S88‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HeLa (cervical) [Cdc25A (human), transfection]
 Cellular systems studied:  cell lines
 Species studied:  human


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