Curated Information
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Curated Information Page
PubMed Id: 10788479 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Kim Y, et al. (2000) Phospholipase D1 is phosphorylated and activated by protein kinase C in caveolin-enriched microdomains within the plasma membrane. J Biol Chem 275, 13621-7 10788479
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S2-p - PLD1 iso2 (rat)
Orthologous residues
PLD1 (human): S2‑p, PLD1 iso2 (human): S2‑p, PLD1 (mouse): S2‑p, PLD1 iso2 (mouse): S2‑p, PLD1 (rat): S2‑p, PLD1 iso2 (rat): S2‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, electrophoretic mobility shift, mutation of modification site
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
 Enzymes shown to modify site in vitro
Type Enzyme
PHOSPHATASE PPP1CA (rat)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol ester increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced

T147-p - PLD1 iso2 (rat)
Orthologous residues
PLD1 (human): T147‑p, PLD1 iso2 (human): T147‑p, PLD1 (mouse): T147‑p, PLD1 iso2 (mouse): T147‑p, PLD1 (rat): T147‑p, PLD1 iso2 (rat): T147‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, electrophoretic mobility shift, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCA (rat)
PHOSPHATASE PPP1CA (rat)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol ester increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced

S561-p - PLD1 iso2 (rat)
Orthologous residues
PLD1 (human): S561‑p, PLD1 iso2 (human): S561‑p, PLD1 (mouse): S561‑p, PLD1 iso2 (mouse): S561‑p, PLD1 (rat): S561‑p, PLD1 iso2 (rat): S561‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, electrophoretic mobility shift, mutation of modification site
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
 Enzymes shown to modify site in vitro
Type Enzyme
PHOSPHATASE PPP1CA (rat)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol ester increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced


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