Curated Information

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PubMed Id: 10514497

This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus^®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus^®, click here.

Gallis B, et al. (1999) Identification of flow-dependent endothelial nitric-oxide synthase phosphorylation sites by mass spectrometry and regulation of phosphorylation and nitric oxide production by the phosphatidylinositol 3-kinase inhibitor LY294002. J Biol Chem 274, 30101-8 10514497

Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.

S116-p - eNOS (cow)

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Orthologous residues

eNOS (human): S114‑p, eNOS (mouse): T113‑p, eNOS (rat): T113‑p, eNOS (rabbit): T120‑p, eNOS (pig): S116‑p, eNOS (cow): S116‑p

Characterization

Methods used to characterize site in vivo: 2D analysis, [32P] bio-synthetic labeling, mass spectrometry, peptide sequencing, phosphoamino acid analysis, phosphopeptide mapping

Relevant cell lines - cell types - tissues: endothelial-aorta

Cellular systems studied: primary cells

Species studied: cow

Upstream Regulation

Treatments, proteins and their effect on site modification:

Treatments	Referenced Treatments	Manipulated Protein	Referenced Protein	Effect	Notes
fluid shear stress				increase

S1179-p - eNOS (cow)

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Orthologous residues

eNOS (human): S1177‑p, eNOS (mouse): S1176‑p, eNOS (rat): S1176‑p, eNOS (rabbit): S1183‑p, eNOS (pig): S1179‑p, eNOS (cow): S1179‑p

Characterization

Methods used to characterize site in vivo: 2D analysis, [32P] bio-synthetic labeling, mass spectrometry, peptide sequencing, phosphoamino acid analysis, phosphopeptide mapping

Relevant cell lines - cell types - tissues: endothelial-aorta

Cellular systems studied: primary cells

Species studied: cow

Enzymes shown to modify site in vitro:

Type	Enzyme
KINASE	Akt1 (human)

Upstream Regulation

Potential in vivo enzymes for site:

Type	Enzyme	Evidence	Notes
KINASE	Akt1 (human)	pharmacological inhibitor of upstream enzyme

Downstream Regulation

Effect of modification (function): enzymatic activity, induced

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