|
Orthologous residues
|
|
Grb2 (human): Y209‑p, Grb2 (mouse): Y209‑p, Grb2 (rat): Y209‑p
|
|
Characterization
|
|
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling, mutation of modification site, phosphoamino acid analysis
|
|
Relevant cell lines - cell types - tissues:
293T (epithelial), A431 (epithelial), BaF3 ('B lymphocyte, precursor'), K562 (erythroid)
|
|
Cellular systems studied:
cell lines
|
|
Species studied:
human, mouse
|
|
Upstream Regulation
|
|
Potential in vivo enzymes for site:
|
|
Type
|
Enzyme
|
Evidence
|
Notes
|
|
KINASE
|
Bcr/Abl (human)
|
genetic transfer of constitutively active upstream enzyme, co-immunoprecipitation, mutation in upstream enzyme recognition motif
|
|
|
|
Treatments, proteins and their effect on site modification:
|
|
Treatments
|
Referenced Treatments
|
Manipulated Protein
|
Referenced Protein
|
Effect
|
Notes
|
|
EGF
|
|
|
|
increase
|
|
|
|
Downstream Regulation
|
|
Effect of modification (function):
molecular association, regulation
|
|
Modification regulates interactions with:
|
|
Interacting molecule
|
Interacting domains
|
Effect
|
Consequences (function)
|
Consequences (process)
|
Detection assays
|
|
SOS1 (human)
|
SH3_1
|
Disrupts
|
|
|
|
|
