Curated Information
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Curated Information Page
PubMed Id: 11551902 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Wick MJ, et al. (2001) Insulin receptor-mediated p62dok tyrosine phosphorylation at residues 362 and 398 plays distinct roles for binding GTPase-activating protein and Nck and is essential for inhibiting insulin-stimulated activation of Ras and Akt. J Biol Chem 276, 42843-50 11551902
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y362-p - Dok1 (human)
Orthologous residues
Dok1 (human): Y362‑p, Dok1 (mouse): Y361‑p, Dok1 (rat): Y361‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, peptide sequencing, phospho-antibody, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial), CHO (fibroblast) [EphB1 (human), transfection]
 Cellular systems studied:  cell lines
 Species studied:  hamster, human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE InsR (human)
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, phosphorylation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Nck1 (human) SH3_1, SH2 Induces co-immunoprecipitation
RASA1 (human) Induces co-immunoprecipitation

Y398-p - Dok1 (human)
Orthologous residues
Dok1 (human): Y398‑p, Dok1 (mouse): Y397‑p, Dok1 (rat): Y397‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, peptide sequencing, phospho-antibody, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial), CHO (fibroblast) [EphB1 (human), transfection]
 Cellular systems studied:  cell lines
 Species studied:  hamster, human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE InsR (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin Grb10 (human) inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, phosphorylation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Nck1 (human) SH3_1, SH2 Induces co-immunoprecipitation
RASA1 (human) Induces co-immunoprecipitation


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