Curated Information
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Curated Information Page
PubMed Id: 11782427 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Lynch DK, Daly RJ (2002) PKB-mediated negative feedback tightly regulates mitogenic signalling via Gab2. EMBO J 21, 72-82 11782427
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S159-p - Gab2 (human)
Orthologous residues
Gab2 (human): S159‑p, Gab2 (mouse): S160‑p, Gab2 (rat): S160‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], MCF-7 (breast cell)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (human) co-immunoprecipitation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
heregulin increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  cell growth, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
HER2 (human) Disrupts co-immunoprecipitation
Shc1 (human) Disrupts co-immunoprecipitation
 Comments:  Introduction of the S159A mutation markedly enhanced HRG-stimulated tyrosine phosphorylation of Gab2. A global amplification of HRG-induced tyrosine phosphorylation was observed in cells expressing S159A Gab2.


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