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Orthologous residues
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Gab2 (human): S159‑p, Gab2 (mouse): S160‑p, Gab2 (rat): S160‑p
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Characterization
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Methods used to characterize site in vivo:
mutation of modification site, phospho-antibody
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Relevant cell lines - cell types - tissues:
293 (epithelial), 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], MCF-7 (breast cell)
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Cellular systems studied:
cell lines
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Species studied:
human, mouse
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Enzymes shown to modify site in vitro:
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Upstream Regulation
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Potential in vivo enzymes for site:
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Type
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Enzyme
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Evidence
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Notes
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KINASE
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Akt1 (human)
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co-immunoprecipitation
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Treatments, proteins and their effect on site modification:
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Treatments
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Referenced Treatments
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Manipulated Protein
|
Referenced Protein
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Effect
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Notes
|
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heregulin
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|
|
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increase
|
|
|
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Downstream Regulation
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Effect of modification (function):
molecular association, regulation
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Effect of modification (process):
cell growth, altered
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Modification regulates interactions with:
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Interacting molecule
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Interacting domains
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Effect
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Consequences (function)
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Consequences (process)
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Detection assays
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|
HER2 (human)
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Disrupts
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|
|
co-immunoprecipitation
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Shc1 (human)
|
|
Disrupts
|
|
|
co-immunoprecipitation
|
|
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Comments:
Introduction of the S159A mutation markedly enhanced HRG-stimulated tyrosine phosphorylation of Gab2. A global amplification of HRG-induced tyrosine phosphorylation was observed in cells expressing S159A Gab2.
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